This product is a one-step RT-PCR kit with high amplification capacity. The RNA-cDNA-PCR reaction is completed continuously in the same reaction system, which is easy and fast to operate, and there is no need to add any reagent in the reaction process, which can effectively reduce the risk of contamination. This product uses the strong extension ability of the Evo M-MLV Reverse transcriptase, integrated hot start Pro Taq The superior performance of HS greatly improves the amplification performance of this product. In order to obtain better amplification results, this product is equipped with two reaction buffers: 2X One-Step Reaction Solution A and 2X One-Step Reaction Solution B. Generally, 2X One-Step Reaction Solution A is used first for RT-PCR amplification, and can be replaced by 2X One-Step Reaction Solution B if the desired amplification results cannot be obtained. If you cannot get the desired amplification result, you can replace it with 2X One-Step Reaction Solution B to try.
| individual parts making up a compound | norm |
| One Step Enzyme Mix*1 | 100 μl |
| 2X One-Step Reaction Solution A*2 | 625 μl x 2 pcs |
| 2X One-Step Reaction Solution B*2 | 625 μl x 2 pcs |
| RNase free water | 1 ml x 2 pcs |
* :: 1: Contains Evo M-MLV RTase, RNase Inhibitor.Pro Taq HS DNA Polymerase .
*2: Contains dNTP Mixture with Reaction Buffer.
Transportation temperature: dry ice or -20℃ ice bag
