This product is a one-step RT-PCR kit with high efficient amplification ability. Reverse transcription and PCR reactions are completed continuously in the same reaction system, which is easy and fast to operate, and there is no need to add any reagent during the reaction process, which can effectively reduce the risk of contamination. Meanwhile, the reaction solution contains the dye required for electrophoresis, and electrophoresis can be carried out directly after the reaction, which saves the labor and time consuming. This product uses the strong extension ability of Evo M-MLV Reverse transcriptase, integrated hot start Pro Taq The superior performance of HS greatly improves the amplification performance of this product.
| individual parts making up a compound | norm |
| One Step Enzyme Mix*1 | 100 μl |
| 2X One-Step Reaction Solution (dye plus)*2 | 625 μl x 2 pc |
| RNase free water | 1 ml x 2 pc |
* :: 1: ContainsEvo M-MLV RTase, RNase Inhibitor.Pro Taq HS DNA Polymerase .
*2: Contains dNTP Mixture with reaction buffer.
Transportation temperature: dry ice or -20℃ ice bag
