SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒

SYBR Green Pro Taq HS Premixed qPCR Kit

Product Code: AG11701

- +

¥1,200

Product Description

It is a 2X premix type kit for qPCR using SYBR Green I chimeric fluorescence method. The reaction solution is very simple to prepare, and only requires the addition of primers, templates and RNase free water. It is a 2X premix reagent that is easy to prepare, requiring only the addition of primers, templates, and RNase free water. It is optimized for SYBR Green I concentration and PCR reaction system, and adopts the superior reaction performance of Pro Taq HS DNA polymerase system, which can effectively inhibit the amplification of non-specific products and improve the PCR amplification efficiency, can carry out Real Time PCR reaction with high sensitivity, and obtain a good standard curve in a wide quantitative region, so as to accurately quantify and detect the target gene.

Product Advantages

①This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out qPCR reaction.
② The product has been optimized for SYBR Green I concentration and PCR reaction system, featuring high amplification efficiency and strong amplification specificity.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix* 1 ml X 5 pcs

*White or yellowish precipitates may form when the solution is stored at -20°C. Dissolve on ice or by hand before use and mix upside down until all precipitates disappear. Do not vortex.

Preservation and transportation
Storage Temperature: -20℃ (Protected from light, long-term storage at -20℃, the product can be stored at 4℃ for 6 months after melting).
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

The product was used for fluorescence quantitative RT-PCR to detect mouse GAPDH The amount of cDNA template added (equivalent to the amount of Total RNA) was 200 ng ~ 0.2 pg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse transcription kit (with gDNA removal reagent, for qPCR) (Code No. AG11705). The results were as follows:

The results are shown above:
①Working curve R2= 0.998, amplification efficiency 99.71 TP3T.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 200 ng ~ 0.2 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

The product was used for fluorescence quantitative RT-PCR for the detection of Human β-Actin The amount of cDNA template added (equivalent to the amount of Total RNA) was 100 ng ~ 1 pg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse transcription kit (with gDNA removal reagent for qPCR) (Code No. AG11705). The results are shown below:

The results are shown above:
①Working curve R2= 1.000, amplification efficiency 99.0%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 100 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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