SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒 II

SYBR Green Pro Taq HS Premix qPCR Kit II

Product Code: AG11702

- +

¥1,200

Product Description

This product is a special reagent for qPCR using SYBR Green chimeric fluorescence method. It is a 2X Premix reagent with easy preparation of reaction solution. The concentration of SYBR Green I and the PCR reaction system have been optimized for high specificity and high PCR amplification efficiency, which enables a highly sensitive Real Time PCR reaction with a good working curve over a wide quantification region for accurate quantitative detection of target genes.

Product Advantages

①This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out qPCR reaction.
② The product has been optimized for SYBR Green I concentration and PCR reaction system, featuring high amplification efficiency and strong amplification specificity.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix II 1 ml X 5 pcs
Preservation and transportation
Storage Temperature: -20℃ (Protected from light, long-term storage at -20℃, the product can be stored at 4℃ for 1 month after melting).
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Two-step fluorescence quantitative RT-qPCR assay using this product Human β Actin The amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 0.1 pg. cDNA was synthesized using our own cDNA synthesizer. Evo M-MLV Reverse transcription reagent premix (for qPCR) (Code No. AG11706). The results are shown below:

The results are shown above:
(i) make a curve R2= 1.000, amplification efficiency 99.0%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 100 ng ~ 0.1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

Two-step fluorescence quantitative RT-qPCR assay for Mouse using this product malat1 The amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 0.1 pg. cDNA was synthesized using our own cDNA synthesizer. Evo M-MLV Reverse transcription reagent premix (for qPCR) (Code No. AG11706). The results are shown below:

The results are shown above:
①Working curve R2= 0.999, amplification efficiency 98.0%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 100 ng ~ 0.1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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