SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒（含ROX）

SYBR Green Pro Taq HS Premixed qPCR Kit (with ROX)

Product Code: AG11718

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¥1,200

Product Description

This product is a special reagent for qPCR using SYBR Green I chimeric fluorescence method, and it is used in SYBR Green Premix. Pro Taq The HS qPCR Kit (AG11701) is based on ROX's 2X Premix reagent, which is convenient to use. In this product, the SYBR Green concentration and the PCR reaction system have been optimized, and the superior reaction performance of the Pro Taq The HS system (mixed with Taq antibody) can effectively inhibit non-specific amplification, improve PCR amplification efficiency, and can obtain a good standard curve in a wide quantitative region for accurate quantitative detection of target genes.

Product Advantages

① This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, just add primers, templates and RNase free water to carry out qPCR reaction.
② The product is optimized for SYBR Green I concentration and PCR reaction system, which can effectively inhibit non-specific amplification and improve PCR efficiency.
③ This product is premixed with ROX Reference Dye, which is convenient to use and effectively reduces the experimental error caused by adding ROX alone.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix (Rox Plus) 1 ml x 5 pc
Preservation and transportation
Storage temperature: -20℃ (keep away from light, long-term storage at -20℃, the product can be stored at 4℃ for 1 month after melting.)
Transportation temperature: dry ice transportation
Example 1

Mouse was detected by two-step fluorescence quantitative RT-PCR using this kit. GAPDH The amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 0.1 pg. cDNA was synthesized using our own cDNA synthesizer. Evo M-MLV Reverse Transcription Kit (for qPCR) (Code. AG11706). The results are as follows:

The results are shown above:
①Working curve R2= 0.999, amplification efficiency 98.41 TP3T.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 100 ng ~ 0.1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

The two-step fluorescence quantitative RT-PCR method using this kit was used for the detection of Human β-Actin The amount of template cDNA added (equivalent to the amount of Total RNA) was 100 ng ~ 1 pg. cDNA was synthesized using the Company's Evo M-MLV Reverse Transcription Kit (for qPCR) (Code. AG11706). The results are as follows:

The results are shown above:
①Working curve R2= 0.999, amplification efficiency 101%.
②Accurate quantification can be performed over a wide template range, and the amplification curve shows good linearity over the range of 100 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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