<i>Pro Taq</i> HS 预混型探针法 qPCR 试剂盒

Pro Taq HS Premixed Probe qPCR Kit

Product Code: AG11704

- +

¥1,200

Product Description

It is a 2X Premix reagent for qPCR using the probe method, and the reaction solution is very easy to prepare. The PCR reaction system of this product has been optimized with the use of the superior reaction performance of the Pro Taq HS system (mixed with Taq antibody), which can effectively inhibit non-specific amplification and improve PCR amplification efficiency, can perform highly sensitive Real Time PCR reactions, and can obtain a good working curve in a wide quantitative region, thus providing accurate quantitative detection of target genes.

Product Advantages

① This product is a 2X premix, the reaction solution is very simple to prepare, only need to add primers, templates, probes and RNase free water can be qPCR reaction.
②This product utilizes a superior performance Pro Taq HS and optimized reaction system with high specificity and amplification efficiency.

Product Composition
individual parts making up a compound norm
2X Pro Taq HS Probe Premix 1 ml X 5 pcs
Preservation and transportation
Storage temperature: -20℃ storage (keep away from light, long-term storage placed at -20℃, the product can be stored at 4℃ for 6 months after melting.)
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

This kit was used for the detection of Human by fluorescent probe qPCR method. β-actin The amount of template cDNA added (equivalent to the amount of Total RNA) was 200 ng ~ 0.2 pg. cDNA synthesis was carried out using our own cDNA synthesizer. Evo M-MLV Reverse transcription kit (with gDNA removal reagent for qPCR) (Code.AG11705). The results were as follows:

The results are shown above:
① The amplification efficiency was 104.81 TP3T and R2= 0.997;
②Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the concentration range of 200 ng ~ 0.2 pg cDNA (equivalent to Total RNA amount).

Example 2

The kit was used for the fluorescent probe qPCR method for the detection of mouse GAPDH The amount of template cDNA added (equivalent to the amount of Total RNA) was 200 ng ~ 0.2 pg. cDNA synthesis was carried out using our own cDNA synthesizer. Evo M-MLV Reverse transcription kit (with gDNA removal reagent for qPCR) (Code. AG11705). The results were as follows:

The results are shown above:
① The amplification efficiency was 104.01 TP3T and R2= 0.997;
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 200 ng ~ 0.2 pg cDNA (equivalent to Total RNA amount).

Related products