This product is a specialized kit for One Step RT-qPCR using the probe method. Reverse transcription and qPCR reactions are completed in the same tube, which is easy and fast to operate and can effectively reduce the risk of contamination. Meanwhile, this product uses the strong extension ability of the Evo M-MLV Reverse transcriptase, integrated hot start Pro Taq With the superior performance of HS, cDNA can be efficiently synthesized and efficiently and stably amplified by qPCR in a short period of time, which is very suitable for the detection of trace RNA such as viral RNA.
① This product is a one-step RT-qPCR reaction kit, completing the reverse transcription and qPCR reaction in a single tube, simplifying the operation, improving efficiency, and effectively reducing the possibility of contamination from multiple operations.
②The product is made of a material with high elongation capacity. Evo M-MLV Reverse transcriptase, high performance hot start enzyme Pro Taq HS and optimized reaction system with high specificity and amplification efficiency.
③This product can rapidly and accurately detect trace amounts of RNA.
④ This product can be used in multiprobe assays.
| individual parts making up a compound | norm |
| 2X One Step RT-qPCR Buffer (Probe)*1 | 1.25 ml x 2 pc |
| Pro Taq HS DNA Polymerase (5 U/μl) | 100 μl |
| Evo M-MLV RTase Enzyme Mix*2 | 100 μl |
| RNase free water | 1 ml x 3 pc |
*1: Contains dNTP Mixture with reaction buffer.
* :: 2: ContainsEvo M-MLV RTase, RNase Inhibitor.
Transportation temperature: dry ice or -20℃ ice pack transportation
