This product can be used to extract total RNA from biological samples such as less than 30 mg of animal tissues (liver, heart, kidney, spleen, intestines, etc.), less than 100 mg of plant tissues (stems, leaves, seeds, etc.), and less than 1.0E+07 cultured cells (HL60, 293T, etc.) etc. The kit adopts a unique cleavage system, which can be used to rapidly lyses the tissues or cells and inhibit the nucleases released from the cells without the need for toxic phenol-chloroform extraction. The unique lysis system eliminates the need for toxic phenol-chloroform extraction and rapidly lyses tissues or cells while inhibiting nuclease released by the cells, maintaining RNA integrity. The genomic DNA is removed by gDNA Eraser Mini Column or DNase I, and then high purity and high yield RNA is obtained by Universal RNA Mini Column, and the purified Total RNA can be directly used in various molecular biology experiments, such as Northern hybridization, mRNA purification, in vitro translation, RT-PCR, and so on. The purified Total RNA can be used directly for Northern hybridization, mRNA purification, in vitro translation, RT-PCR and other molecular biology experiments. If desired, Total RNA can be treated with DNase I (Code No. AG12001) to remove trace amounts of DNA.
| individual parts making up a compound | norm |
| 50X DTT Solution*1 | 700 μl |
| DNase I (RNase free)*1 | 200 μl |
| 10X DNase I Buffer*1 | 1 ml |
| Buffer RLS | 15 ml × 2 |
| Buffer RWA | 30 ml |
| Buffer RWB*2 | 27 ml |
| RNase Free Water*3 | 13 ml |
| gDNA Eraser Mini Columns | 50 sets |
| Universal RNA Mini Columns | 50 sets |
| Collection tubes | 50 pcs |
| RNase Free Tubes*4 | 50 pcs |
*1 These three components are packaged in Package 2-1 and should be stored at -20°C. The remaining components are packaged in Package 2-2 and should be stored at room temperature (15 - 25°C).
* 2 Buffer RWB Before first use, add 63 ml of 100% ethanol (3:7 Buffer RWB to anhydrous ethanol by volume), mix well, label the bottle and store at room temperature.
*3 Recommended storage at -20°C after opening.
*4 This component is for elution of RNA only; centrifuge tubes for pre-lysis should be provided.
| Sample Name | Grinding method | sample size (of a liquid or gas) | Amount received (μg) |
|---|---|---|---|
| mouse liver | Tissue Homogenizer | 10 mg | ~60 |
| mouse heart | Tissue Homogenizer | 10 mg | ~15 |
| mouse spleen | Tissue Homogenizer | 10 mg | ~32 |
| mouse lung | Tissue Homogenizer | 10 mg | ~13 |
| mouse kidney | Tissue Homogenizer | 10 mg | ~18 |
| mouse brain | Tissue Homogenizer | 10 mg | ~8 |
| mouse intestine | Tissue Homogenizer | 10 mg | ~32 |
| mouse stomach | Tissue Homogenizer | 10 mg | ~14 |
| mouse muscle | Tissue Homogenizer | 10 mg | ~4 |
| mouse tail | Tissue Homogenizer | 10 mg | ~5 |
| mouse ear | Tissue Homogenizer | 10 mg | ~17 |
| mouse thymus | Tissue Homogenizer | 5 mg | ~13 |
| mouse pancreas | Tissue Homogenizer | 10 mg | ~11 |
| mouse fat | Tissue Homogenizer | 10 mg | ~3 |
| mouse eyeball | Tissue Homogenizer | 5 mg | ~4 |
Preservation Temperature:
Package 2-1 -20°C Storage;
Package 2-2 Store at room temperature (15-25°C).
Transportation temperature:
Package 2-1 Dry ice or -20°C ice packs for transportation;
Package 2-2 Room Temperature Transportation.
