This product can be used to extract total RNA from 50~100 mg of tissue material (roots, stems, leaves, flowers, fruits, seeds) of various plants (simple plants and complex plants rich in polysaccharides and polyphenols, starch, oils, etc.) and fungi, etc. The kit adopts a unique lysis system, which does not require toxic phenol-chloroform extraction, and it can inhibit the nuclease released by the cells and maintain the integrity of the RNA while lysing the tissues rapidly. It can rapidly lyses the tissue while inhibiting the nuclease released by the cells and maintaining the integrity of RNA, and at the same time creates suitable conditions for the adsorption of RNA onto silica gel membrane. The residual genomic DNA is removed by DNase I, and then washed with two different buffers to remove impurities such as salt, protein and macromolecules, and finally eluted with RNase Free Water to obtain high purity and high yield of RNA.
This product contains two different lysis systems: the Simple Plant Lysis System and the Complex Plant Lysis System. The Simple Plant Lysis System provides a lysis buffer, Buffer RLS, which is composed of guanidine isothiocyanate and is suitable for lysis of most plant tissues. Due to the special secondary metabolites of some plants, guanidine isothiocyanate is prone to precipitate the samples, resulting in poor RNA extraction, then we can try to use the complex plant lysis system, which removes polysaccharides, polyphenols, starch, oils and other complex components under the action of lysis buffers PPS and PA-2, and then lyses the plant tissues through Buffer RLS. Tissue lysis by Buffer RLS. The RNA obtained by using this product is of high purity, with less contamination of protein, genomic DNA and other impurities, and can be directly used in RT-PCR, Real Time RT-PCR, construction of cDNA libraries, Northern hybridization, spotted hybridization, Poly A screening, in vitro translation, RNA degradation enzyme protection analysis, molecular cloning, microarray analysis and other molecular biology experiments. Experimentation.
| individual parts making up a compound | norm |
| 50X DTT Solution*1 | 700 μl |
| DNase I (RNase free)*1 | 200 μl |
| 10X DNase I Buffer*1 | 1 ml |
| Buffer PPS | 25 ml |
| Buffer PA-2 | 1.5 ml X 2 |
| Buffer RLS | 15 ml x 2 |
| Buffer RWA | 30 ml |
| Buffer RWB*2 | 30 ml |
| RNase Free Water*3 | 13 ml |
| Plant RNA Mini Columns*4 | 50 sets |
| Collection tubes | 50 pcs |
| RNase Free Tubes*4 | 50 pcs |
*1 These three components are packaged in Package 2-1 and should be stored at -20°C. The remaining components are packaged in Package 2-2 and should be stored at room temperature (15 - 25°C).
*2 Before using Buffer RWB for the first time, please add 70ml of 100% ethanol (Buffer RWB to anhydrous ethanol volume ratio of 3:7), mix well, mark the bottle and store it at room temperature.
*3 Recommended storage at -20°C after opening.
*4 This component is for elution of RNA only; centrifuge tubes for pre-lysis should be provided.
Preservation Temperature:
Package 2-1 -20°C Storage;
Package 2-2 Store at room temperature (15-25°C).
Transportation temperature:
Package 2-1 Dry ice or -20°C ice packs for transportation;
Package 2-2 Room Temperature Transportation.
