Our HST08 receptor cells are prepared from E. coli HST08 strain by a special process and can be used for DNA chemical transformation. E. coli HST08 Yes mrr, hsdRMS, mcrBC, mcrA These genes are essential for E. coli to excise exogenously methylated DNA, which makes them widely used for transformation of methylation plasmids, preparation of gene libraries, and subcloning. When using the pUC series of plasmids for DNA transformation, recombinant strains can be identified by blue and white spot screening using β-galactosidase activity (α-complementarity). Transformation efficiency is > 5×10% with pUC19 plasmid DNA transformation.8 cfu / μg.
| individual parts making up a compound | norm |
| E. coli HST08 Competent cells* | 100 μl x 10 pc |
| SOC Medium | 1 ml × 10 pc |
| pUC19 plasmid (0.1 ng / μl) | 10 μl |
Suitable for plasmid transformation, gene cloning, blue and white spot screening and other uses.
Storage temperature: -80℃
Transportation temperature: dry ice transportation
