<i>Evo M-MLV</i> 反转录预混型示踪试剂盒（含去除 gDNA 试剂，用于qPCR，黄色）

Evo M-MLV Reverse transcription premixed tracer kit (with gDNA removal reagent for qPCR, yellow)

Product Code: AG11734

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¥1,500

Product Description

This product utilizes M-MLV Reverse transcription kits for reverse transcription polymerase. The cDNA product is ready for qPCR. The 5X gDNA Clean Reaction Mix removes residual genomic DNA and ensures accurate quantitative results. Evo M-MLV RT Reaction Mix (Yellow) contains a yellow dye for visualization of reverse transcription pipetting to avoid missing reagents, and contains all the components required for reverse transcription reactions, optimized for Oligo dT (18T) Primer, Random 6 mers Primer dosage, making the reverse transcription products compatible with chimeric and probe-based methods. qPCR analysis, enabling efficient gene expression analysis.

5X Evo M-MLV RT Reaction Mix (Yellow) contains a yellow dye, the cDNA obtained by reverse transcription is yellow in color and can be used with different quantification reagents:

①Can be used with SYBR Green Premix with blue dye. Pro Taq HS qPCR Tracking Kit (Code No. AG11733) can be used to avoid pipetting errors and visualize the template pipetting process: add the cDNA (yellow) obtained from the reverse transcription of the product to the blue qPCR reaction solution, the solution will turn green, and you can confirm whether to add the template according to the color change, which is conducive to the addition of a large number of samples and reduces the probability of misoperation. It is favorable for the addition of a large number of samples and reduces the probability of misoperation.
② Can be used with our other quantitative reagents [e.g., SYBR Green Premix]. Pro Taq HS qPCR Kit (Code No. AG11701)], when yellow cDNA is added to the colorless quantification reagent, it will change to light yellow and can be visualized by pipetting. However, if the amount of cDNA stock solution added is small, the color change is not obvious and may not be discernible.
③ The performance of quantitative products of other companies used with this product has not been verified, if you need to use, please test the performance first.

Product Advantages

① This product contains a yellow dye, which enables pipetting visualization when reversed.
② The cDNA obtained by reverse transcription is yellow in color: it can be used with our blue SYBR Green Premix. Pro Taq HS qPCR Tracking Kit (Code No. AG11733) can be used to visualize the template pipetting process, avoid pipetting errors, improve the efficiency of sampling and reduce the probability of misuse. It can also be used with our other quantitative reagents.
③ Genomic DNA Removal Reagent 5X gDNA Clean Reaction Mix is included to quickly remove genomic DNA from RNA templates.
④ This kit will remove the genome and reverse transcription system separately formulated into a premix, easy to use, can reduce the loss of reagents and experimental errors.
⑤ The cDNA obtained from this product can be used for both SYBR and probe qPCR analysis.

Product Composition
individual parts making up a compound norm
5X gDNA Clean Reaction Mix 200 μl
5X Evo M-MLV RT Reaction Mix (Yellow)*1 400 μl
RNase free water 1 ml X 2 pcs

*1: contains Evo M-MLV RTase, RNase Inhibitor, dNTPs, Oligo dT (18T) Primer, Random 6 mers Primer, and yellow dye.

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Mouse liver Total RNA (100 ng ~ 1 pg) was used as a template, and the genomic DNA was removed using this kit, then reverse transcription was performed, and using this cDNA as a template, 2 μl of the cDNA stock solution was taken, and the cDNA stock solution was extracted using SYBR Green Premix. Pro Taq HS qPCR Tracking Kit (Code No. AG11733) was used for qPCR amplification detection. APOE 1 Genes. The quantitative instrument used: CFX96 Real-Time PCR Detection System. the results were as follows:

The results are shown above:
① Working curve R2= 0.998 with an amplification efficiency of 99.91 TP3T;
② This product has a high reversal efficiency and can be accurately quantified over a wide template range. The cDNA amplification curves obtained by reverse transcription of 100 ng ~ 1 pg Total RNA show good linearity.
③ The melting curve has a single peak type and good amplification specificity.

Example 2

Using 293T cell Total RNA (100 ng ~10 pg) as a template, the genomic DNA was removed using this kit, and then reverse transcription was performed, and using this cDNA as a template, 2 μl of the cDNA stock solution was taken, and the cDNA stock solution was extracted using SYBR Green Premix. Pro Taq HS qPCR Tracking Kit (Code No. AG11733) for qPCR amplification detection. β-actin Genes. The quantitative instrument used: CFX96 Real-Time PCR Detection System. the results were as follows:

The results are shown above:
① Working curve R2= 0.998 and the amplification efficiency was 93.41 TP3T.
② This product has a high reversal efficiency and can be accurately quantified over a wide template range. The cDNA amplification curves obtained by reverse transcription of 100 ng ~ 10 pg Total RNA show good linearity.
③ The melting curve has a single peak type and good amplification specificity.

 

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