L-Exp Taq DNA polymerase (Mg)2++, dNTPs+)

Product Code: AG11415

- +

¥240

Product Description
L-Exp Taq DNA Polymerase is synthesized on our superior performance Accurate Taq The addition of high-fidelity enzymes to the enzyme results in partial 3′-5′ Exonuclease activity (Proof reading activity), making it ideal for PCR amplification of long and complex DNA. Most of the PCR products obtained with this product have an A base at the 3’ end and can be cloned directly into T vectors.
This product is specially optimized to provide not only very strong long fragment amplification performance, but also the ability to amplify complex templates, using Human genomic DNA as a template can amplify ~24 kb DNA fragments, and the amplification success rate is high for the difficult to amplify templates with high GC content (~75%).
Product Advantages
L-Exp Taq DNA Polymerase with the addition of high-fidelity enzymes relative to the Accurate Taq Has higher fidelity and stronger amplification ability. High amplification efficiency and low mismatch rate under normal PCR amplification conditions.
② This product has very strong long fragment amplification performance, amplifying ~40 kb of DNA fragments using λDNA as a template and ~24 kb of DNA fragments using Human genomic DNA as a template.
③ Suitable for amplification of complex templates such as DNA fragments with high GC content.
Product Composition
individual parts making up a compound norm
L-Exp Taq DNA Polymerase (5 U/μl) 25 μl
5X L- Exp Taq PCR Buffer (Mg)2+ plus) 500 μl
dNTP Mix (10 mM each) 100 μl
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag transportation
Example 1

Using Human gDNA as a template, this kit amplifies DNA fragments of different lengths, and it can amplify 24 kb DNA fragments very well.

The electrophoresis results are shown below:

Example 2

Using λDNA as a template, the kit amplifies DNA fragments of different lengths, and is able to amplify 40 kb of DNA fragments.

The electrophoresis results are shown below: