SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒（含示踪染料，含 Rox）

SYBR Green Pro Taq HS Premixed qPCR Kit (with Tracer Dye, with Rox)

Product Code: AG11735

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¥1,200

Product Description

This product is a special reagent for qPCR using SYBR Green chimeric fluorescence method. 2X Premix is pre-mixed with ROX Reference Dye, and the final concentration of ROX in the reaction is 0.08 μM, which is convenient to use without additional addition. Meanwhile, 2X Premix is equipped with a blue dye, which is paired with a yellow template dilution buffer [40X Dilution Buffer (Yellow)] to visualize the pipetting process: after mixing the yellow dilution buffer with the template and adding it to the blue reaction solution, the solution will change from blue to green; you can confirm the addition of the template based on the change in color, which is conducive to the addition of a large number of samples and reduces the probability of mishandling. Reduce the probability of misoperation.
In this product, the SYBR Green I concentration and PCR reaction system have been optimized, and the superior reaction performance of the Pro Taq HS system (mixed with Taq antibody), which can effectively inhibit non-specific amplification, improve PCR amplification efficiency, and can obtain a good working curve in a wide quantitative region for accurate quantitative detection of target genes.

Product Advantages

① Blue dye is added to 2X Premix with yellow template diluent to visualize the pipetting process, improve the efficiency of sampling, and reduce the probability of mishandling.
② 2X SYBR Green Pro Taq HS Premix (Blue, Rox Plus) is premixed with ROX Reference Dye for ease of use and to effectively minimize experimental errors associated with the addition of ROX alone.
③This product is a 2X premixed solution, pre-mixed with SYBR. Green I, the reaction solution is very simple to prepare, only need to add primers, templates and RNase free water can be qPCR reaction.
④ This product is effective for SYBR The concentration of Green I and the PCR reaction system were optimized for high amplification efficiency and high amplification specificity.

 

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix (Blue, Rox Plus) 1 ml X 5 pc
40X Dilution Buffer (Yellow) 500 μl
Preservation and transportation
Storage temperature: -20℃ (keep away from light)
Transportation temperature: -20℃ Ice bag transportation or dry ice transportation
Example 1

The product was used for fluorescence quantitative RT-PCR to detect the presence of Mouse. GAPDH Gene (GC content 56.1%), and the amount of cDNA template added (equivalent to the amount of Total RNA) was 10 ng ~ 100 fg. The cDNA template was prepared using the Company's Evo M-MLV Reverse transcription premix kit (with gDNA removal reagent for qPCR) (Code No. AG11728) to synthesize cDNA. quantification instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
①Working curve R2= 0.9996, amplification efficiency 103.51 TP3T.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 10 ng ~ 100 fg concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

Fluorescence quantitative RT-PCR method using this product for detection of Human TFR GThe amount of template cDNA added (equivalent to the amount of Total RNA) was 10 ng ~ 1 pg. The amount of template cDNA added was 10 ng ~ 1 pg using our Evo M-MLV Reverse transcription premix kit (with gDNA removal reagent for qPCR) (Code No. AG11728) to synthesize cDNA. quantification instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
①Working curve R2= 0.9992, amplification efficiency 104.71 TP3T.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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