SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒 III（含高 Rox）

SYBR Green Pro Taq HS Premixed qPCR Kit III (with High Rox)

Product Code: AG11738

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¥1,200

Product Description

It is a 2X premix for qPCR using SYBR Green I chimeric fluorescence method. The reaction solution is very simple to prepare, and it is easy to use because it only requires the addition of primers, templates, and RNase free water for qPCR reaction. The product is optimized for SYBR Green concentration and PCR reaction system, and adopts the superior reaction performance of the Pro Taq HS system, which can effectively inhibit the amplification of non-specific products and improve the efficiency of PCR amplification, and at the same time, with high fluorescence signal value, it can get good amplification in a wide quantitative region, thus achieving accurate detection.
This product is supplemented with a high concentration of ROX dye, and the final concentration of ROX in the reaction is 0.4 μM. It is suitable for use in quantitative PCR instruments that are calibrated with a high concentration of ROX, or in quantitative PCR instruments that do not require calibration.

 

Product Advantages

① This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, just add primers, templates and RNase free water to carry out qPCR reaction.
② The product is optimized for SYBR Green I concentration and PCR reaction system, with high fluorescence signal value, high amplification efficiency and strong amplification specificity.
③ This product is premixed with ROX Reference Dye, which is convenient to use and effectively reduces the experimental error caused by single addition of ROX. It is suitable for high concentration ROX calibrated quantitative PCR instruments or quantitative PCR instruments that do not need to be calibrated.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix III (High Rox Plus) 1 ml x 5 pc
Preservation and transportation
Storage temperature: -20℃ (keep away from light)
Transportation temperature: dry ice or -20℃ ice pack transportation
Example 1

The cDNA was used as the template, and the amount of template added (equivalent to the amount of Total RNA) was 50 ng~500 fg. The kit was used for fluorescence quantitative PCR to detect the presence of cDNA in mice and to detect the presence of cDNA in mice. GAPDH The cDNA was synthesized using our own Evo M-MLV Reverse transcription premix kit (with gDNA removal reagent, for qPCR) (Code. AG11728).
Quantification instrument used: ABI StepOne Real-Time PCR System. results are as follows:

The results are shown above:
① Working curve R2= 0.9998, amplification efficiency 102%.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 50 ng ~ 500 fg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

This kit was used for the fluorescence quantitative RT-PCR detection of Human β-Actin The amount of cDNA template added (equivalent to Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse transcription premix kit (with gDNA removal reagent, for qPCR) (Code. AG11728).
The quantification instrument used: Bio-Rad CFX96 Real-Time PCR System. results were as follows:

The results are shown above:
①Working curve R2= 0.9978, amplification efficiency 93.01 TP3T.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

 

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