SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒 III（含低Rox）

SYBR Green Pro Taq HS Premixed qPCR Kit III (with Low Rox)

Product Code: AG11739

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¥1,200

Product Description

It is a 2X premix for qPCR using SYBR Green I chimeric fluorescence method. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out the qPCR reaction, which is easy to use.
The product is optimized for SYBR Green I concentration and PCR reaction system, and adopts the superior reaction performance of the Pro Taq HS system, which can effectively inhibit the amplification of non-specific products and improve the efficiency of PCR amplification, and at the same time, with high fluorescence signal value, it can get good amplification in a wide quantitative region, thus achieving accurate detection.
This product has a low concentration of ROX dye added, and the final concentration of ROX in the reaction is 0.04 μM, which is suitable for quantitative PCR instruments calibrated with a low concentration of ROX, or quantitative PCR instruments that do not need to be calibrated.

 

Product Advantages

①This product is a 2X premixed solution, premixed with SYBR Green I. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out qPCR reaction.
② The product is optimized for SYBR Green I concentration and PCR reaction system, with high fluorescence signal value, high amplification efficiency and strong amplification specificity.
③ This product is premixed with ROX Reference Dye, which is convenient to use and effectively reduces the experimental error caused by single addition of ROX. It is suitable for low concentration ROX calibrated quantitative PCR instruments, or quantitative PCR instruments that do not require calibration.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix III (Low Rox Plus) 1 ml X 5 pcs
Preservation and transportation
Storage temperature: -20℃ (keep away from light)
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

The product was used for fluorescence quantitative RT-PCR to detect mouse GAPDH The amount of cDNA template added (equivalent to the amount of Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728).
Quantification instrument used: Bio-Rad CFX96TM Real-Time PCR Detection Systems. the results are shown below:

The results are shown above:
① Working curve R2= 0.9986, amplification efficiency 90%.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

The product was used for fluorescence quantitative RT-PCR for the detection of Human β-Actin The amount of cDNA template added (equivalent to the amount of Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728).
Quantitative instrument used: ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
①Working curve R2= 0.9967, amplification efficiency 99.0%.
②Accurate quantification can be performed over a wide template range, with amplification curves showing good linearity over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

 

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