This product is available from OD600 Various endotoxin-free plasmids (Plasmid, Cosmid, etc.) were extracted from culture broth of ≤ 600 (e.g., broth volume of 300 ml when the OD value of the broth is 2.0). The optimized alkaline lysis method, combined with anion exchange resin and unique endotoxin removal system, can effectively remove endotoxin, protein and other impurities, and then desalted and concentrated by isopropanol precipitation, and finally dissolved in Endo-free TE Buffer or Endo-free water to obtain high yield and high purity of the plasmid DNA. Endotoxin content < 0.1 EU/μg, can be directly used in transfection, in vitro transcription and translation, enzyme modification and other molecular biology experiments. In the same quality of the bacterium, the plasmid copy number (high copy or low copy), purification of the plasmid DNA yield is different. In general, if extracting high copy plasmid (e.g. pUC19, etc.), it is recommended to use 100 ~ 200 ml of overnight culture medium, which can purify 200 ~ 500 μg of high purity plasmid DNA; if extracting low copy plasmid (e.g. pBR322, etc.), it is recommended to use 200 ~ 300 ml of overnight culture medium, which can purify 100 ~ 150 μg of high purity plasmid DNA. plasmid DNA.
| individual parts making up a compound | norm |
| RNase A (10 mg/ml)*2 | 1.2 ml |
| Buffer PEL | 100 ml |
| Buffer PRS*2 | 120 ml |
| Buffer PLS | 100 ml |
| Buffer PBS | 100 ml |
| Buffer ER | 30 ml |
| Buffer PWB | 250 ml |
| Endo-free Elution Buffer | 100 ml |
| Endo-free water for 70% ethanol*3 | 18 ml |
| Endo-free TE Buffer | 10 ml |
| Column Filter | 10 pcs |
| Plasmid DNA Midi Columns | 10 pcs |
| RNase Free Tubes | 10 pcs |
* 1: RNase A (10 mg / ml) is packed in Package 2-1 and should be stored at -20℃; the rest of the components are packed in Package 2-2 and should be stored at room temperature (15 ~ 30℃).
*2: Before the first use of Buffer PRS, RNase A should be added to Buffer PRS (RNase A to Buffer PRS volume ratio is 1:100), mixed well and labeled on the bottle. After adding RNase A, the Buffer PRS should be stored at 2~8℃ for 6 months.
*3: Endo-free water for 70% ethanol Prior to first use, 42 ml of your own anhydrous ethanol should be drawn up and added to make 70% ethanol.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Storage at room temperature (15 ~ 30°C)
Transportation temperature:
Package 2-1 Dry Ice Transportation or -20℃ Ice Pack Transportation
Package 2-2 Room Temperature (15 ~ 30°C) Transportation
