It is a one-step RT-PCR kit with high amplification capacity, integrating the superior reaction performance of the Evo M-MLV Reverse transcriptase and Pro Taq HS DNA polymerase, formulated into Premix type One Step Enzyme Mix Ⅱ, reverse transcription and PCR reactions are completed in the same reaction system, the operation is simple and fast, the reaction process does not need to add any reagents, which can effectively reduce the risk of contamination.
This product is widely applicable to different templates, with strong amplification efficiency and high detection sensitivity, which makes it easier to analyze the amplification of RNA samples. At the same time, this product is added with electrophoresis dye, which can be used for electrophoretic detection directly after reaction, and can get the detection results faster.
1. This is a one-step RT-PCR reaction kit, which can complete the reverse transcription and PCR reactions in a single tube, simplifying the operation, improving the efficiency, and effectively reducing the possibility of contamination caused by multiple operations.
2. This product is characterized by wide applicability to different templates, high detection sensitivity and strong amplification specificity.
3. This product contains an electrophoresis indicator, which can be used for electrophoresis directly after the PCR reaction, and there is only one indicator band in electrophoresis, which is light green in color.
| individual parts making up a compound | norm |
| One Step Enzyme Mix II | 100 μl |
| 2X One-Step Reaction Solution II (dye plus) | 625 μl x 2 pcs |
| RNase free water | 1 ml x 2 pcs |
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
