This product is a rapid identification of sheep using a probe method.*1 Kit for components of animal origin. Identification of animal-derived components based on interspecies 16S rRNA (16S ribosomal RNA) gene polymorphisms. The product contains three probes labeled with different fluorescent moieties (FAM, VIC, ROX), which allow simultaneous detection of sheep-derived 16S rRNA genes in the same reaction tube.*2The eukaryotic 18S rRNA (18S ribosomal RNA) gene was used as a key component of the eukaryotic gene.*3 and Internal Control, which allows you to monitor whether a reaction is proceeding properly. Detection of the 18S rRNA gene allows monitoring of the quality of eukaryotic genomic DNA extraction.
The 2X Master Mix for Ovine has already mixed DNA polymerase, dNTP Mixture and reaction buffer, the reaction solution is very simple to prepare, just add the Primer Mix for Ovine, Probe Mix for Ovine, RNase free water and the sample to be examined. It is easy to prepare the reaction solution, just add the Primer Mix for Ovine, Probe Mix for Ovine, RNase free water and the sample to be tested.
The PCR reaction system of this product has been optimized with the use of a superior reaction performance Pro Taq The HS system (mixed with Taq antibody) can effectively inhibit non-specific amplification, and together with the carefully optimized buffer, it improves PCR amplification efficiency and enables highly sensitive Real Time PCR reactions.
* 1: This product can detect common sheep breeds, such as common sheep ( Ovis aries ), certain breeds of sheep may not be accurately detected because of possible variation in the sequences of different breeds.
*2: The 16S rRNA gene in this product can specifically detect sheep-derived components, and the primer probe sequences are referenced from the Agricultural Industry Standard of the People's Republic of China NY/T 3309-2018.
*3: The 18S rRNA gene in this product can specifically detect eukaryotic organisms, and the primer probe sequences are referenced from the National Standard of the People's Republic of China GB/T 38164-2019.
① This product combines DNA polymerase, dNTP Mixture and reaction buffer into one tube, which is easy and quick to operate, and can effectively reduce the risk of sample contamination caused by experimental operations and shorten the time required for experiments.
②This product utilizes a superior performance Pro Taq HS and optimized reaction system with high specificity and amplification efficiency.
③ This product can simultaneously detect sheep-derived 16S rRNA gene, eukaryotic 18S rRNA gene and Internal Control in one tube, and can specifically identify sheep-derived components.
| individual parts making up a compound | norm |
| 2X Master Mix for Ovine*1 | 625 μl |
| Primer Mix for Ovine*2 | 50 μl |
| Probe Mix for Ovine*3 | 50 μl |
| Control DNA for Ovine*4 | 25 μl |
| RNase free water | 1 ml |
*1: 2X Master Mix for Ovine contains Pro Taq HS DNA Polymerase, dNTP Mixture and reaction buffer, etc.
*2: Primer Mix for Ovine contains sheep-derived 16S rRNA gene, eukaryotic 18S rRNA gene, primers for Internal Control assay and Internal Control template.
*3: Probe Mix for Ovine contains sheep-derived 16S rRNA gene, eukaryotic 18S rRNA gene, and probes for Internal Control assay.
*4: Control DNA for Ovine is a plasmid template containing only sequences specific to sheep-derived components.
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
