<i>AccuLyo</i> Taq HS 预混型可冻干探针法 qPCR 试剂盒（含 UNG）

AccuLyo Taq HS Premixed Lyophilizable Probe-Based qPCR Kit (with UNG)

Product Code: AG11757

- +

¥1,400

Product Description

This product is a 2X Premix premix with lyophilization protectant, which can be used directly for lyophilization preparation. The premix can also be lyophilized by adding primers and probes, and the lyophilized product is easy to be stored and transported at room temperature.
This product contains the superior reaction performance of Accurate Taq HS DNA polymerase, together with carefully optimized reaction buffer, improves PCR amplification performance and sensitivity of low concentration template detection, enabling highly sensitive Real Time qPCR reactions for accurate quantitative detection of target genes.
The dUTP/UNG anti-contamination system is introduced in this product. dUTP replaces dTTP in the PCR reaction, and the UNG enzyme selectively hydrolyzes dU-containing DNA strands without affecting non-dU-containing DNA strands, thus removing dU-containing contaminating templates introduced in the preparation of the PCR reaction system, and thus preventing the generation of false-positive results in the PCR reaction effectively; At the same time, monoclonal antibody that can inhibit the activity of DNA Polymerase at room temperature is also added to this product, which can effectively inhibit non-specific amplification, improve the sensitivity of the reaction and enhance the accuracy of the results.

Product Advantages

① This product is a 2X Premix premix with lyophilization protection agent, which can be used directly for lyophilization preparation; the premix can also be lyophilized by adding primers and probes, and the lyophilized product is easy to be stored and transported at room temperature.
②This product utilizes a superior performance Accurate Taq HS DNA polymerase and optimized reaction system with high specificity and amplification efficiency.
The dUTP/UNG Contamination Prevention System has been introduced to remove contamination of dU-containing PCR products, thereby preventing false-positive PCR results and improving the accuracy of experimental results.

Product Composition
individual parts making up a compound norm
2X AccuLyo Taq HS Probe Premix (UNG Plus) 1 ml X 5 pcs

 

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation

Human cDNA (100 ng ~ 1 pg) was used as a template, and RNase free water was used as a negative control to replace the template amount. The performance of the lyophilized and untreated lyophilized products placed at 37℃ for 30 days was tested in triplicate for the TFR gene (GC content 38%, FAM channel), Actin gene (GC content 59%, HEX channel), and RPP30 gene (GC content 62%, Cy5 channel). TFR gene (GC content 38%, FAM channel), Actin gene (GC content 59%, HEX channel) and RPP30 gene (GC content 62%, Cy5 channel) were tested in a triple assay at the same time. The performance of the freeze-dried product placed at 37℃ for 30 days (green curve) is comparable to that of the untreated lyophilized product (red curve).

The quantitative instruments used for the assay were ABI QuantStudioTM 5 Real-Time PCR Systems. the results of the triple test are shown below:

[FAM channel]


The results are shown above:
1、 Accurate quantification can be carried out in a wide range of templates, and the amplification curves of qPCR reactions with 100 ng - 1 pg cDNA show good linearity.
2. Negative controls were not detected within 45 cycles.
3. The performance of the lyophilized product was comparable to that of the untreated product after being placed at 37℃ for 30 days.

[HEX Channel]


The results are shown above:
1、Accurate quantification can be carried out in a wide range of templates, and the amplification curves of qPCR reactions with 100 ng - 1 pg cDNA show good linearity;
2. Negative controls were not detected within 45 cycles.
3. The performance of the lyophilized product was comparable to that of the untreated product after being placed at 37℃ for 30 days to amplify the gene.

[Cy5 channel]


The results are shown above:
1、Accurate quantification can be carried out in a wide range of templates, and the amplification curves of qPCR reactions with 100 ng - 1 pg cDNA show good linearity;
2. Negative controls were not detected within 45 cycles.
3. The performance of the lyophilized product was comparable to that of the untreated product after being placed at 37℃ for 30 days to amplify the gene.

Related products