This is a specialized kit for one-step RT-qPCR based on the probe method. Reverse transcription and qPCR reactions are completed in the same tube, which makes the operation simple and fast.
This product utilizes a high extension capacity Evo M-MLV Reverse transcriptase, integrated hot start Accurate Taq With the superior performance of HS, cDNA can be synthesized in a short period of time and amplified by qPCR with high efficiency and stability. The product is widely applicable to the amplification of genes of different complexity, and can be used for single or multiplexed detection. It is suitable for the detection of viral RNA and other trace RNA.
The dUTP/UNG anti-contamination system is introduced in this product. dUTP replaces dTTP in the PCR reaction, and the UNG enzyme selectively hydrolyzes dU-containing DNA strands without affecting non-dU-containing DNA strands, thus removing dU-containing contaminating templates introduced in the preparation of the PCR reaction system, and thus preventing the generation of false-positive results in the PCR reaction effectively; At the same time, monoclonal antibody that can inhibit the activity of DNA Polymerase at room temperature is also added to this product, which can effectively inhibit non-specific amplification, improve the sensitivity of the reaction and enhance the accuracy of the results.
1. This is a one-step RT-qPCR reaction kit, which can complete the reverse transcription and qPCR reaction in a single tube, simplifying the operation, improving the efficiency, and effectively reducing the possibility of contamination caused by multiple operations.
2. This product adopts reverse transcriptase and hot-start polymerase with superior reaction performance, together with carefully optimized reaction system, featuring high specificity and amplification efficiency.
3. dUTP/UNG is a contamination prevention system that removes contamination of dU-containing PCR products to prevent false-positive PCR results and improve the accuracy of the results.
| individual parts making up a compound | norm |
| 2X One Step RT-qPCR Buffer IV (Probe) | 1.25 ml X 2 pcs |
| One Step Enzyme Mix IV* | 400 μl |
| RNase free water | 1 ml X 3 pcs |
*1: contains Evo M-MLV RTase Enzyme,Accurate Taq HS DNA Polymerase, UNG Enzyme and RNase Inhibitor, etc.
Transportation temperature:Dry ice transportation or -20°C ice pack transportation.
