Product Description
This is a dual fluorescent staining kit for cell viability based on Calcein AM and EthD-1. Calcein AM and EthD-1 can be used to stain live cells and dead cells respectively, based on the principle that Calcein AM is highly hydrophobic and can easily penetrate the cell membrane to enter the cell, and then hydrolyzed by esterase to Calcein in the cell (Ex / Em = 494 nm / 517 nm). Calcein AM has strong hydrophobicity and can easily penetrate the cell membrane and enter the cell, after entering the living cell, it is hydrolyzed by esterase to Calcein and stays in the cell, generating a strong green fluorescence (Ex/Em = 494 nm/517 nm); EthD-1 is a high-affinity nucleic acid fluorescent dye, which can't pass through the membrane of the living cell but can pass through the membrane of the dead cell and reach the cell nucleus, and binds with nucleic acid of the dead cell to generate a strong red fluorescence (Ex/Em = 528 nm/617 nm). show green fluorescence and dead cells show red fluorescence.
This product is easy to operate, only need to be stained for 20~30 min and then can be used for fluorescence microscopy, flow cytometry analysis, laser confocal microscopy analysis, enzyme labeling or other fluorescence detection system analysis; Wide range of application, can be used for the detection of most of the eukaryotic animal cells, but is not applicable to bacteria and yeast.
This product is easy to operate, only need to be stained for 20~30 min and then can be used for fluorescence microscopy, flow cytometry analysis, laser confocal microscopy analysis, enzyme labeling or other fluorescence detection system analysis; Wide range of application, can be used for the detection of most of the eukaryotic animal cells, but is not applicable to bacteria and yeast.
Product Advantages
1. The product is easy to operate, can be tested at room temperature or 37℃, and can be stained for 20~30 min for testing.
2. Calecin AM has low cytotoxicity and generally does not inhibit cell function.
3. EthD-1 has better nucleic acid binding properties and higher luminescence intensity than PI (propidium iodide).
4. It can be used for the detection of a variety of instruments, such as flow cytometry, fluorescence microscopy, laser confocal microscopy and enzyme labeling.
2. Calecin AM has low cytotoxicity and generally does not inhibit cell function.
3. EthD-1 has better nucleic acid binding properties and higher luminescence intensity than PI (propidium iodide).
4. It can be used for the detection of a variety of instruments, such as flow cytometry, fluorescence microscopy, laser confocal microscopy and enzyme labeling.
Product Composition
| individual parts making up a compound | AG51007 ( 50 rxns*1 ) | AG51008 ( 150 rxns*1 ) |
| Calcein AM (4 mM) | 12.5 μl | 37.5 μl |
| EthD-1 (2 mM) | 50 μl | 150 μl |
*1: The number of reactions in this kit is calculated as one reaction of 500 μl of working solution in one well of a 12-well plate for fluorescence microscopy. The number of reactions for different detection methods is calculated in the following table:
*2: The recommended reagent dosages in the table may be adjusted according to actual conditions.
Preservation and transportation
Storage temperature: -20℃ keep away from light
Transportation temperature: -20℃ ice bag transportation
Transportation temperature: -20℃ ice bag transportation
