Product Description
This product is a Whole Genome Amplification (WGA) kit for non-discriminatory whole genome amplification (WGA) of 1~1000 Cells or 10 pg~10 ng genomic DNA. Based on the isothermal amplification system of Multiple Displacement Amplification (MDA), amplification is performed under the action of Phi29 DNA polymerase, and the size of the amplification products obtained from the whole genome of a single cell ranges from 2 to 100 kb, with an average product size of about 15 kb, which can be widely used for genome and exon sequencing, large fragment copy number sequencing, and other applications. It can be widely used for whole genome and exon sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis and gene chip analysis.
Phi29 DNA polymerase is used in this product, which has strong amplification performance, strong strand displacement activity and strand affinity. With optimized Buffer system, it can amplify DNA with high coverage in 2~2.5 h, which is also suitable for samples with complex structure. Meanwhile, Phi29 DNA polymerase has 3’-5’ exonuclease activity, which ensures high fidelity and accuracy of DNA synthesis.
Phi29 DNA polymerase is used in this product, which has strong amplification performance, strong strand displacement activity and strand affinity. With optimized Buffer system, it can amplify DNA with high coverage in 2~2.5 h, which is also suitable for samples with complex structure. Meanwhile, Phi29 DNA polymerase has 3’-5’ exonuclease activity, which ensures high fidelity and accuracy of DNA synthesis.
Product Advantages
1. Cell lysis and genomic DNA denaturation at room temperature (15~25℃) reduces the number of DNA breaks and improves the homogeneity of whole-genome amplification compared with denaturation at high temperature.
2. Based on the high fidelity, ultra-high DNA polymerization activity and strand replacement activity of Phi29 DNA polymerase, longer amplified fragments (2~100 kb) can be obtained, which is conducive to the improvement of single-cell whole genome coverage.
3. High sensitivity, non-selective amplification of the whole genome at the level of single cells and 10 pg of genomic DNA, and low amplification preference, resulting in accurate, well-homogenized, high-coverage amplified genomic sequences.
4. Simple and fast operation, constant temperature (30 ℃) reaction for 2 h, can obtain a large number of DNA.
2. Based on the high fidelity, ultra-high DNA polymerization activity and strand replacement activity of Phi29 DNA polymerase, longer amplified fragments (2~100 kb) can be obtained, which is conducive to the improvement of single-cell whole genome coverage.
3. High sensitivity, non-selective amplification of the whole genome at the level of single cells and 10 pg of genomic DNA, and low amplification preference, resulting in accurate, well-homogenized, high-coverage amplified genomic sequences.
4. Simple and fast operation, constant temperature (30 ℃) reaction for 2 h, can obtain a large number of DNA.
Product Composition
| individual parts making up a compound | AG12516 | AG12517 |
| Buffer DLS*1 | 250 μl | 1 ml |
| Stop Reaction Buffer*2 | 200 μl | 800 μl |
| DTT ( 1 M )*1 | 50 μl | 200 μl |
| AccuNext WGA Polymerase*3 | 24 μl | 96 μl |
| AccuNext WGA Auxiliary Enzyme | 30 μl | 120 μl |
| 4X WGA Reaction Buffer | 150 μl | 600 μl |
| Nuclease free water | 1 ml | 1 ml X 2 pcs |
*1: For amplification of genomic DNA, Buffer DLS should be prepared as Buffer D1; for amplification of cells, Buffer DLS and DTT should be prepared as Buffer D2. Please refer to<操作方法>。
*2: For amplification of genomic DNA, the Stop Reaction Buffer should be prepared as Buffer N1; for cell amplification, the Stop Reaction Buffer should be used directly.<操作方法>。
*3: This component contains Phi29 DNA polymerase, avoid repeated freezing and thawing.
Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: -20℃ Ice bag transportation or dry ice transportation
Transportation temperature: -20℃ Ice bag transportation or dry ice transportation
