<i>GenDiff</i> SNP 预混型 SYBR 法 qPCR 试剂盒（ARMS）

GenDiff SNP Premixed SYBR qPCR Kit (ARMS)

Product Code: AG12802

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¥1,400

Product Description

This kit is a combination of ARMS-PCR and SYBR Green I chimeric fluorescence method for SNP genotyping. It is a 2X pre-mixed reagent that requires only primers, template, and RNase free water, making the preparation of the reaction solution very simple. The product is optimized for SYBR Green I concentration and PCR reaction system, which can amplify templates of different complexity; the product uses modified Taq DNA Polymerase, which has high amplification efficiency and sensitivity, and monoclonal antibody that can inhibit the activity of DNA Polymerase at room temperature has been added. At the same time, a monoclonal antibody that can inhibit the activity of DNA Polymerase at room temperature is also added, which can effectively inhibit the formation of primer dimer and non-specific amplification, and it can carry out highly sensitive Real Time PCR reaction, which is very suitable for the detection of SNP genotyping.

 

Product Advantages

1. It is a 2X premixed solution, easy to prepare, only need to add primers, templates and RNase free water to carry out qPCR reaction.
2. The results of this product are easy to analyze. Genotyping can be performed by analyzing the CT difference between two genotypes, which is simple to analyze and the results are reliable.
3. This product is characterized by high sensitivity, high amplification efficiency, excellent typing performance, easy interpretation of results and good versatility.

Product Composition
individual parts making up a compound norm
2X SYBR qPCR Mix (ARMS)*1 1 ml X 5 pcs

*1: White or yellowish precipitate may occur when the solution is stored at -20°C. Dissolve on ice or by hand before use and mix upside down until all precipitate disappears. Do not vortex.

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Use this product for fluorescence quantitative PCR in mice. GAPDH genes, the template was added at 100 ng - 1 pg, and cDNA was synthesized using the company's Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728). The quantification instruments used were ABI QuantStudioTM 5 Real-Time PCR Systems. the results are shown below:

The results are shown above:
1. Working curve R2= 0.999, amplification efficiency 100%;
2. Accurate quantification is possible over a wide template range, with good linearity in the amplification curve over the 100 ng - 1 pg concentration range;
3. The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

ABI QuantStudio is used for the quantification of different genotypes (wild type, mutant) of different samples.TM 5 Real-Time PCR Systems, different types of templates can be accurately differentiated by comparing the differences in Ct values, and the results are as follows:

The results are shown above:
1. In the typing result 1, according to the amplification curve, it was found that the wild-type template amplified normally, and the mutant template did not have peaks, which could distinguish the mutant type from the wild-type significantly;
2. In typing result 2, the difference between mutant and wild-type Ct was found to be ≥ 14 according to the amplification curve, which can significantly distinguish the mutant from the wild-type.

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