Product Description
This product is a DNA library construction kit developed for illumina's high-throughput sequencing platform, which can prepare sequencing libraries from fragmented 100 pg ~ 1 μg double-stranded DNA samples. The product adopts optimized Buffer system, which improves the steps of end repair/dA tail addition, junction ligation and library amplification to enhance the ligation and amplification efficiency; it is widely applicable to a wide range of samples, which can ensure the success of library construction to a large extent. This product should be used in conjunction with illumina sequencing junctions and PCR amplification primers for library construction. AccuNext Short Adapter for DNA Library Kit ( Illumina) (Code No. AG12537 / AG12538) and AccuNext CDI Oligos for DNA Library Kit ( Illumina) (Code No. AG12529 / AG12530 / AG12531)], you can obtain DNA libraries suitable for sequencing on the illumina platform.
The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change the dosage and concentration of any reaction components or replace the components in this product with other equivalent products, in order to avoid obtaining bad experimental results. If replacement is needed, please verify it first.
The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change the dosage and concentration of any reaction components or replace the components in this product with other equivalent products, in order to avoid obtaining bad experimental results. If replacement is needed, please verify it first.
Product Advantages
1. Single-tube end-repair, with dA tailing, significantly reduces experiment time.
2. Compatible with enzymatically and mechanically interrupted DNA fragments.
3. Efficiently repairs the ends of fragmented DNA, with the repair product containing a phosphate group at the 5’ end and a dA tail at the 3’ end.
4. Wide range of application, can be used for library construction of 100 pg - 1 μg DNA samples after fragmentation.
2. Compatible with enzymatically and mechanically interrupted DNA fragments.
3. Efficiently repairs the ends of fragmented DNA, with the repair product containing a phosphate group at the 5’ end and a dA tail at the 3’ end.
4. Wide range of application, can be used for library construction of 100 pg - 1 μg DNA samples after fragmentation.
Product Composition
| individual parts making up a compound | AG12535 | AG12536 |
| End Repair Enzyme Mix | 24 μl | 96 μl |
| 10X End Repair Buffer | 72 μl | 288 μl |
| T4 DNA Ligase (350 U/μl) | 12 μl | 48 μl |
| Ligation Buffer | 300 μl | 1.2 ml |
| 2X AccuNext PCR Mix III | 300 μl | 1.2 ml |
| Nuclease free water | 1 ml X 2 pcs | 1 ml X 5 pcs |
*The connectors for illumina sequencing and primers for PCR amplification required for connector attachment are necessary for the experiment but are not configured in this product and need to be purchased separately. For example, you can use AccuNext Short Adapter for DNA Library Kit ( Illumina) (Code No. AG12537 / AG12538) and AccuNext CDI Oligos for DNA Library Kit ( Illumina) (Code No. AG12529 / AG12530 / AG12531)].
Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: -20℃ ice bag transportation
Transportation temperature: -20℃ ice bag transportation
