<i>ChiDigit</i> 探针法芯片式数字 PCR 试剂盒

ChiDigit Probe-based Digital PCR Kit on Chip

Product Code: AG12901

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¥1,200

Product Description

This is a 2X Premix Probe DNA Assay Kit for Chip digital PCR (cdPCR). The reaction solution is very simple and quick to prepare, only need to add primers, probes, templates and RNase free water to carry out the PCR reaction, which can effectively reduce the risk of contamination.
This product adopts the superior reaction performance Pro Taq The HS system (mixed with Taq antibody) inhibits non-specific amplification, increases reaction sensitivity and improves accuracy. This product contains ROX dye for quality control of the effective number of microwells.

 

Product Advantages

1. This is a 2X Premix Probe DNA Detection Kit, the preparation of reaction solution is very simple and fast, only need to add primers, probes, templates and RNase free water to carry out the digital PCR reaction, which can effectively reduce the possibility of contamination due to multiple operations.
2. This product adopts hot-start polymerase with superior reaction performance, together with carefully optimized reaction system, featuring high specificity and amplification efficiency.

Product Composition
individual parts making up a compound norm
2X Chip dPCR Premix for Probe *1 500 μl X 2 pcs
RNase free water 1 ml

*1: contains Pro Taq HS DNA Polymerase, dNTP Mixture, ROX and Reaction Buffer, etc.

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice pack transportation.

(ABI QuantStudio™ Absolute Q™ Digital PCR System as an example)

Example 1

The product was used to amplify the African swine fever (ASFV) standard (theoretical detection copy number of 4500 copies), and the experimental results were as follows:

The results are shown above:

1, QC QC effective holes for 20477, to meet the quality control requirements (quality control requirements for the total number of effective microporous greater than 20000);
2. Positive micropores are evenly dispersed and no positive micropores are aggregated;
3. The yin and yang partitions are clear, which helps in the setting of the threshold line;
4. The actual amount of templates detected was 4274 copies, which is consistent with the theoretical number of copies.

Example 2

The product was used to amplify Human TFR gene, the theoretical amount of DNA template added were 100 copies, 10 copies, 1 copy, 0 copy (negative control), the experimental results are as follows:

The results are shown above:
1, QC QC effective holes for 20400, to meet the quality control requirements (quality control requirements for the total number of effective microporous greater than 20000);
2. Positive micropores are evenly dispersed and no positive micropores are aggregated;
3. The yin and yang partitions are clear, which helps in the setting of the threshold line;
4. The actual amount of templates detected was 96 copies, 7 copies, 1 copy and 0 copy respectively, which was consistent with the theoretical copy number.

Example 3

The product was used to amplify Human CEPH The genes were analyzed for copy number variation, and a four-channel assay was performed, with the FAM, VIC, ABY and JUN channels each detecting one locus (the theoretical ratio of detected copy number for each channel, FAM : VIC : ABY : JUN = 1:1:1:2), and the results were as follows:

The results are shown above:
1, QC QC effective holes for 20458, to meet the quality control requirements (quality control requirements for the total number of effective microporous greater than 20000);
2. Positive micropores are evenly dispersed and no positive micropores are aggregated;
3. The yin and yang partitions are clear, which helps in the setting of the threshold line;
4. Quadruple testing is available;
5. The number of copies detected by different fluorescence channels meets FAM : VIC : ABY : JUN ≈ 1:1:1:2, which is consistent with the theoretical copy number.

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