SYBR Green <i>Pro Taq</i> HS 预混型 qPCR 试剂盒（高GC）

SYBR Green Pro Taq HS Premix qPCR Kit (High GC)

Product Code: AG11759

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¥1,200

Product Description

This is a 2X premix kit for qPCR using SYBR Green I chimeric fluorescence method. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water. This product has optimized the SYBR Green I concentration and PCR reaction system, and added relevant auxiliary proteins, which greatly improves the amplification performance of complex templates (such as GC content greater than 60%), and also has good amplification performance for ordinary GC content templates. Pro Taq HS DNA polymerase system, which can effectively inhibit the amplification of non-specific products and improve the PCR amplification efficiency, can carry out Real Time PCR reaction with high sensitivity, and obtain a good standard curve in a wide quantitative region, so as to accurately quantify and detect the target gene.

 

Product Advantages

1. This product is a 2X premix, pre-mixed with SYBR Green I. The reaction solution is very simple to prepare, only need to add primers, templates and RNase free water to carry out qPCR reactions.
2. The product is optimized for SYBR Green I concentration and PCR reaction system, and auxiliary proteins are added to greatly improve the amplification performance of complex templates (e.g., GC content greater than 60%).
3. This product has high detection sensitivity, can get good standard curve in a wide quantitative region, accurate quantitative detection of target genes, good reproducibility and high confidence.

Product Composition
individual parts making up a compound norm
2X SYBR Green Pro Taq HS Premix (GC rich)* 1 ml X 5 pcs

*: White or yellowish precipitate may occur when the solution is stored at -20°C. Dissolve on ice or by hand before use and mix upside down until all precipitate disappears. Do not vortex and oscillate.

Preservation and transportation
Storage temperature: -20℃ storage (keep away from light)
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Quantitative fluorescence assay using this product Human WNT5A （The amount of cDNA template added (equivalent to the amount of Total RNA) was 100 ng ~ 10 pg. cDNA was synthesized using the cDNA template of our Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent, for qPCR) Ver.2 (Code No. AG11728 ). The results were as follows:

The results are shown above:
① Working curve R2= 0.9990, amplification efficiency 103.21 TP3T.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 100 ng ~ 10 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

Fluorescence quantitative detection of mice using this product GAPDH （The amount of cDNA template added (equivalent to Total RNA) was 10 ng ~ 1 pg. cDNA was synthesized using the Company's Evo M-MLV Reverse transcription premix kit (with gDNA removal reagent, for qPCR) Ver.2 (Code No. AG11728 ). The results were as follows:

The results are shown above:
① Working curve R2= 0.9995, amplification efficiency 93.2% .
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 10 ng ~ 1 pg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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