This product is a recombinantly expressed source of phage SP6 RNA polymerase from E. coli, which is a DNA-dependent RNA polymerase with a molecular weight of 98.5 kDa.RNA synthesized by SP6 RNA polymerase is suitable for a variety of scientific research and biotechnology applications. The enzyme uses double-stranded DNA containing the SP6 promoter sequence as a template and NTP as a substrate to synthesize RNA that is complementary to the single-stranded DNA downstream of the promoter, which is highly specific for the SP6 promoter sequence and does not recognize promoters from other biological sources.The sequence of the SP6 promoter is as follows, ATTTAGGTGACACTATAGAAGNG, where G is the first base of transcription. base of transcription.
| individual parts making up a compound | norm |
| SP6 RNA Polymerase ( 50U/μl ) | 100 μl |
| 10X SP6 Transcription Buffer | 1 ml |
| RNase free water | 1 ml |
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
