This product is a phage T7 RNA polymerase from a recombinant expression source in E. coli. It uses double-stranded DNA containing the T7 promoter sequence (5’-TAATACGACTCACTATAG*-3’) as a template, and NTP as a substrate, to synthesize RNA that is complementary to the reversed single-stranded DNA downstream of the promoter; The enzyme is highly specific for the T7 promoter sequence and does not recognize promoters from other biological sources. The double-stranded linear flat-end or 5’ protruding DNA can be used as substrate templates for T7 RNA polymerase, so that linear plasmids and PCR products can be used as templates for in vitro RNA synthesis.
Note: G* is the first base of RNA transcription, including this base, the target sequence with 3 consecutive G, in vitro transcription efficiency can be significantly improved, according to the experimental needs of the design.
| individual parts making up a compound | norm |
| T7 RNA Polymerase ( 100 U/μl ) | 100 μl |
| 10X T7 Transcription Buffer | 1 ml |
| RNase free water | 1 ml |
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
