2X Accurate Taq Premix (with dye) II

Product Code: AG11022

- +

¥120

Product Description
this product is Accurate Taq  DNA polymerase ready-to-use 2-fold PCR reaction premix, containing Accurate Taq DNA Polymerase, dNTPs and optimized Buffer system, PCR reaction, just add the template, primers and water to the premixed solution can be amplified. At the same time, the product is also added with the coloring reagent required for electrophoresis testing, the solution of the product is purple-red in color, and it can be used for agarose gel electrophoresis directly after the PCR reaction is completed. The premixed solution is easy to use, minimizes human error, reduces the risk of multi-step contamination, and provides results in a short period of time. The PCR product has an A base at the 3’ end and can be cloned directly into T vectors.
Product Advantages
①This product is a 2X premix, only need to add the template and primers to the premix for amplification, easy to operate, can minimize human error, reduce the possible contamination caused by multi-step operation, and the test results can be obtained in a relatively short period of time.
②2X Accurate Taq Master Mix (dye plus) II has good amplification performance, amplifying ~12 kb of DNA fragments using λDNA as a template and ~4 kb of DNA fragments using Human genomic DNA as a template.
③This product contains violet dye, after PCR can be directly carried out agarose gel electrophoresis, without adding electrophoresis sample buffer, electrophoresis has a light violet-red indicator band.
Product Composition
individual parts making up a compound norm
2X Accurate Taq Master Mix (dye plus) II 500 μl x 6 pc
RNase free water 1 ml x 3 pc
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag
Example 1

The gDNA and λDNA of Human, chicken heart and tomato species were used as templates, and different lengths of DNA fragments were amplified using this kit, and good amplification results were obtained.

The electrophoresis results are shown below:

Example 2

The Human gDNA was used as the template, and this kit was used to amplify DNA fragments with high AT content and high GC content, and the amplification results were very good.

The electrophoresis results are shown below:

Example 3

Human gDNA is used as a template to amplify 2 kb DNA fragments with different template amounts (500 ng, 200 ng, 100 ng, 10 ng, 1 ng, 100 pg). Target fragments can be amplified with template amounts as low as 1 ng.

The electrophoresis results are shown below:

table of examples of experiments
Template Type Amplification speed Fragment size (kb) Sequence GC content Withdrawal method template volume Can it be amplified
Chicken gDNA 1 min / kb 0.5 kb 41.0% Magnetic bead method 74 ng
Chicken gDNA 1 min / kb 1.2 kb 36.6% Magnetic bead method 74 ng
Chicken gDNA 1 min / kb 1.7 kb 68.6% Magnetic bead method 74 ng
Chicken gDNA 1 min / kb 2.4 kb 49.4% Magnetic bead method 74 ng
Human gDNA 30 sec /kb 0.5 kb 46.2% reagent method 100 ng
Human gDNA 1 min / kb 0.5 kb 35.7% reagent method 100 ng
Human gDNA 1 min / kb 0.5 kb 75.4% reagent method 100 ng
Human gDNA 30 sec /kb 1 kb 36.1% reagent method 100 ng
Human gDNA 30 sec /kb 2 kb 38.1% reagent method 100 ng
Human gDNA 1 min / kb 2.1 kb 26.9% reagent method 100 ng
Human gDNA 1 min / kb 3 kb 38.7% reagent method 100 ng
Human gDNA 1 min / kb 3.4 kb 39.2% reagent method 100 ng
Human gDNA 1 min / kb 4 kb 36.0% reagent method 100 ng
Human gDNA 1 min / kb 4 kb 38.2% reagent method 100 ng
Mouse gDNA 1 min / kb 1 kb 52.2% Magnetic bead method 100 ng
Mouse gDNA 1 min / kb 3.4 kb 53.4% Magnetic bead method 100 ng
Pig gDNA 1 min / kb 0.6 kb 47.6% Magnetic bead method 30 ng
Pig gDNA 1 min / kb 2 kb 57.9% Magnetic bead method 30 ng
Tomato gDNA 1 min / kb 0.5 kb 28.2% Magnetic bead/column method 120 ng
Tomato gDNA 1 min / kb 1.6 kb 32.2% Magnetic bead/column method 120 ng
Tomato gDNA 1 min / kb 1.9 kb 36.0% Magnetic bead/column method 120 ng
Tomato gDNA 1 min / kb 3 kb 37.4% Magnetic bead/column method 120 ng
λDNA 1 min / kb 12 kb 51.6% - 2 ng
Escherichia coli colony 1 min / kb 1 kb 53.8% / single colony
Escherichia coli colony 1 min / kb 2 kb 51.1% / single colony
Escherichia coli colony 1 min / kb 3.5 kb 52.2% / single colony