2X Pro Taq Premix Ver.2

Product Code: AG11107

norm

120 rxns / 50 μl

Clear

- +

¥200

Product Description

This product is ready to use Pro Taq Enzyme PCR reaction 2-fold pre-mix containing Pro Taq DNA Polymerase, dNTPs, and optimized Buffer systems are used to amplify the PCR reaction by simply adding template, primers, and water to the premix. This premix solution is easy to use, minimizes human error, reduces contamination caused by multiple steps, and results can be obtained in a short period of time. The Pro Taq enzyme utilizes our superior performance. Accurate Taq The enzyme is enriched with a high-fidelity enzyme with partial 3′→5′ Exonuclease activity (Proof reading activity), which is ideal for amplification of DNA fragments larger than 10 kb with good fidelity, and the PCR product has an A base at the 3’ end, which allows it to be cloned directly into a T vector.

Product Advantages

① The product is a 2X premix, only need to add template, primer and water to the premix for amplification, easy to operate, can minimize human error, reduce the possible contamination caused by multi-step operation, and the results can be obtained in a relatively short period of time.
② 2X Pro Taq Master Mix Ver.2 is thermally stable and can amplify ~40kb of DNA fragments using λDNA as a template and ~17.5kb of DNA fragments using Human genomic DNA as a template.

③High-fidelity enzyme is added to this product, relative to the Accurate Taq Master Mix has higher fidelity. High amplification efficiency and low mismatch rate under normal PCR amplification conditions.

Product Composition

individual parts making up a compound	AG11107
2X Pro Taq Master Mix Ver.2	500 μl x 6 pc
RNase free water	1 ml x 3 pc

Preservation and transportation

Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag

Example 1

Using λDNA as a template, a 40 kb DNA fragment can be amplified very well.

The electrophoresis results are shown below:

Example 2

Using human genomic DNA as a template, we were able to amplify DNA fragments up to 17.5 kb in length.

The electrophoresis results are shown below:

Example 3

The gDNA of chicken blood and pig blood were used as templates to amplify 10 kb of DNA fragments, and the target fragments were well amplified.

The electrophoresis results are shown below:

Example 4

A 15 kb DNA fragment was amplified using rice and soybean gDNA as templates, and the target fragments were well amplified.

The electrophoresis results are shown below:

Example 5

Using λDNA as template and adding different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg), the kit was used to amplify 2 kb DNA fragments with templates as low as 100 fg, and the target fragments could be amplified.