SYBR Green <i>SupTaq</i> HS 预混型 qPCR 试剂盒（含高 Rox）

SYBR Green SupTaq HS Premixed qPCR Kit with High Rox

Product Code: AG11761

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¥1,200

Product Description

It is a 2X premix for qPCR using SYBR Green I chimeric fluorescence method. The reaction solution is very simple to prepare and can be amplified by adding primers, template and RNase free water.
This product uses mutant Taq DNA polymerase, which is suitable for rapid PCR reaction, and monoclonal antibody, which can inhibit the activity of DNA polymerase at room temperature, to realize rapid detection and effectively inhibit the amplification of non-specific products. At the same time, the product has been optimized for SYBR Green concentration and PCR reaction system, with high fluorescence signal value and excellent amplification efficiency, which can be well amplified in a wide quantitative region to achieve accurate detection.
With the addition of ROX dye, the final concentration of ROX in the reaction is 0.4 μM, which is suitable for quantitative PCR instruments calibrated at high concentrations of ROX, or quantitative PCR instruments that do not require calibration.

 

Product Advantages

1、This product uses a mutant Taq DNA polymerase for fast and accurate quantitative detection of target genes.
2、This product is a 2X premixed liquid, easy to formulate;
3. The product is optimized for SYBR Green I concentration and PCR reaction system, with high fluorescence signal value, high amplification efficiency and strong amplification specificity.
4、This product is premixed with ROX Reference Dye, which is convenient to use and effectively reduces the experimental error caused by single addition of ROX. It is suitable for high concentration ROX calibrated quantitative PCR instruments or quantitative PCR instruments that do not need to be calibrated.

Product Composition
individual parts making up a compound norm
2X SYBR Green SupTaq HS Premix (High Rox Plus) 1 ml X 5 pcs

 

Preservation and transportation
Storage temperature: -20℃ (keep away from light)
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

The cDNA was used as the template, and the amount of template added (equivalent to the amount of Total RNA) was 100 ng ~ 10 fg. The product was used for fluorescence quantitative PCR to detect the presence of cDNA in mice. ApoE The cDNA was synthesized using our own Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728).
Quantification instrument used: ABI StepOne Real-Time PCR System Plus. results were as follows:

The results are shown above:
① Working curve R2= 0.9995, amplification efficiency 99%.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 100 ng ~ 100 fg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

The product was used for fluorescence quantitative RT-PCR to detect mouse ApoE The amount of cDNA template added (equivalent to the amount of Total RNA) was 100 ng ~ 10 fg. cDNA was synthesized using our own cDNA template. Evo M-MLV Reverse Transcription Premix Kit (with gDNA removal reagent for qPCR) Ver.2 (Code No. AG11728).
The quantification instrument used: Bio-Rad CFX96 Real-Time PCR System. results were as follows:

The results are shown above:
① Working curve R2= 0.9995, amplification efficiency 101%.
② Accurate quantification can be performed over a wide template range, with good linearity in the amplification curve over the range of 100 ng ~ 10 fg cDNA concentration (equivalent to Total RNA amount).
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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