This product is a kit for synthesizing 1st Strand cDNA from RNA using reverse transcriptase and contains all the reagents required for 1st Strand cDNA synthesis. This product uses reverse transcriptase Evo M-MLV Plus Reverse Transcriptase with strong cDNA elongation. The product is available in Evo M-MLV Reverse Transcriptase has been modified on the basis of Reverse Transcriptase, which is very suitable for the reverse transcription of RNA containing complex secondary structures or long-stranded RNA. This product can inhibit the non-specific binding and extension of reverse transcriptase to RNA with complex structure, and thus obtain full-length cDNA with good specificity and integrity, which is suitable for cDNA library construction and full-length cDNA synthesis.
| individual parts making up a compound | norm |
| Evo M-MLV Plus RTase (200 U/μl) | 50 μl |
| 5X RTase Plus Reaction Buffer | 200 μl |
| RNase Inhibitor (40 U/μl) | 25 μl |
| dNTP Mix (10 mM each) | 50 μl |
| Oligo dT (18T) Primer (50 μM) | 50 μl |
| Random 6 mers Primer (50 μM) | 100 μl |
| RNase free water | 1 ml |
Transportation temperature: dry ice or -20℃ ice bag
