This product is available from OD600 The plasmid DNA can be extracted from culture broth with OD ≤ 200 OD (e.g., the volume of broth is 100 ml when the OD value of the broth is 2.0), and the plasmid DNA can be extracted without endotoxin. 250 μg of endotoxin-free plasmid DNA can be extracted from plasmid by optimized alkaline lysis method using silica membrane adsorption technology combined with the unique endotoxin removing system and rinsing system, which can remove the endotoxin, proteins and other impurities effectively while extracting the plasmid. The purified plasmid DNA has high yield and low endotoxin content, which can be directly used in transfection, in vitro transcription and translation, enzyme modification and other molecular biology experiments.
| individual parts making up a compound | norm |
| RNase A (10 mg/ml) | 300 μl |
| Buffer PRS-2*2 | 30 ml |
| Buffer PLS-2 | 25 ml |
| Buffer PBS-2 | 25 ml |
| Buffer ER | 8 ml |
| Buffer PWA | 30 ml |
| Buffer PWB-2*3 | 30 ml |
| Endo-free Elution Buffer | 10 ml |
| Plasmid DNA Maxi Columns | 10 sets |
| RNase Free Tubes | 10 pcs |
*1: RNase A (10 mg/ml) is packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and should be stored at room temperature (15 ~ 30℃).
*2: Before the first use of Buffer PRS-2, it is necessary to add RNase A into Buffer PRS-2 (the volume ratio of RNase A to Buffer PRS-2 is 1:100), mix it well, and then mark the bottle. Buffer PRS-2 with RNase A should be stored at 2~8℃ for 6 months.
*3: Before using Buffer PWB-2 for the first time, please add 70 ml of anhydrous ethanol (the volume ratio of Buffer PWB-2 to anhydrous ethanol is 3:7), mix well, mark the bottle and store at room temperature.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Storage at room temperature (15 ~ 30°C)
Transportation temperature:
Package 2-1 Dry Ice or -20°C Ice Pack Transportation
Package 2-2 Room Temperature Transportation
