This product is available from OD600 The plasmid DNA can be extracted from culture broth with OD ≤ 600 OD (e.g., the volume of broth is 300 ml when the OD value of broth is 2.0) to obtain as much as 1 mg of endotoxin-free plasmid DNA.The product adopts optimized alkaline lysis method and silica membrane adsorption technology combined with unique endotoxin removal system and rinsing system, which can effectively remove endotoxin, protein and other impurities while extracting the plasmid. The purified plasmid DNA has high yield and low endotoxin content, which can be directly used in transfection, in vitro transcription and translation, enzyme modification and other molecular biology experiments.
| individual parts making up a compound | norm |
| RNase A (10 mg/ml) | 1.2 ml |
| Buffer PRS-2*2 | 120 ml |
| Buffer PLS-2 | 100 ml |
| Buffer PBS-2 | 100 ml |
| Buffer ER | 30 ml |
| Buffer PWA | 80 ml |
| Buffer PWB-2*3 | 75 ml |
| Endo-free Elution Buffer | 20 ml |
| Plasmid DNA Ultra Columns | 10 sets |
| RNase Free Tubes | 10 pcs |
| Column Filter | 10 pcs |
*1: RNase A (10 mg/ml) is packaged in Package 2-1 and should be stored at -20℃; the rest of the components are packaged in Package 2-2 and should be stored at room temperature (15 ~ 30℃).
*2: Before the first use of Buffer PRS-2, it is necessary to add RNase A into Buffer PRS-2 (the volume ratio of RNase A to Buffer PRS-2 is 1:100), mix it well, and then mark the bottle. Buffer PRS-2 with RNase A should be stored at 2~8℃ for 6 months.
*3: Before using Buffer PWB-2 for the first time, please add 175 ml of anhydrous ethanol (the volume ratio of Buffer PWB-2 to anhydrous ethanol is 3:7), mix it well, mark the bottle and store it at room temperature.
Preservation Temperature:
Package 2-1 -20°C Storage
Package 2-2 Storage at room temperature (15 ~ 30°C)
Transportation temperature:
Package 2-1 Dry Ice or -20°C Ice Pack Transportation
Package 2-2 Room Temperature Transportation
