Exp Taq DNA Polymerase (with GC Buffer)

Product Code: AG11511

norm

50 rxns / 50 μl

Clear

- +

¥280

Product Description

This product is designed for the amplification of GC-rich templates. The DNA Polymerase and reaction buffer have been optimized for the amplification of GC-rich or repetitive genomic DNA and cDNA templates, and it is characterized by a low mismatch rate and a high level of amplification performance. Most PCR products amplified with this product have an A base at the 3’ end and can be cloned directly into T vectors.
In order to obtain better amplification results, this product is equipped with two reaction buffers: 2X GC buffer I and 2X GC buffer II; in general, it is recommended to use 2X GC buffer I for PCR amplification first, and then use 2X GC buffer II if you cannot get the desired results.

Product Advantages

① This product is a PCR amplification product specially designed for high GC content templates. The DNA Polymerase and reaction buffer have been optimized, and it is very suitable for the amplification of genomic DNA and cDNA templates with complex structures, GC-rich sequences, or containing repetitive sequences.
Most PCR products have an A base at the 3’ end and can be cloned directly into T vectors.
③ Amplification of high GC genes with different types of templates has good performance.

Product Composition

individual parts making up a compound	norm
Exp Taq DNA Polymerase (5 U/μl)	25 μl
2X GC buffer I	625 μl x 2 pc
2X GC buffer II	625 μl x 2 pc
dNTP Mix (10 mM each)	100 μl

Preservation and transportation

Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag

Example 1

Human gDNA was used as a template to amplify DNA fragments with different GC contents using 2X GC buffer I and 2X GC buffer II of the kit, respectively.

The electrophoresis results are shown below:

Example 2

Mouse gDNA was used as a template, and DNA fragments with different GC contents were amplified using 2X GC buffer I and 2X GC buffer II of this kit, respectively.

The electrophoresis results are shown below:

Example 3

Porcine gDNA was used as a template to amplify DNA fragments with different GC contents using 2X GC buffer I and 2X GC buffer II of the kit.

The electrophoresis results are shown below:

Example 4

Chicken or maize gDNA was used as templates to amplify DNA fragments with different GC contents using 2X GC buffer I and 2X GC buffer II of this kit, respectively.

The electrophoresis results are shown below:

Example 5

Using mouse gDNA as template, the 2X GC buffer I of the kit was used to amplify 1658 bp DNA fragments (GC content 65%) and the 2X GC buffer II was used to amplify 1166 bp DNA fragments (GC content 70.8%) by adding different amounts of templates (500 ng, 100 ng, 10 ng, and 1 ng), and the target fragments could be amplified at template amounts as low as 1 ng. Target fragments were amplified at template levels as low as 1 ng.