This is a colorimetric protein quantification kit based on the BCA method (Bicinchoninic Acid), which is based on the principle that in an alkaline environment, the peptide bonds in proteins can reduce Cu²⁺ to Cu⁺, and the resulting Cu⁺ reacts with the BCA reagent to form a stable blue-purple The resulting Cu⁺ reacts with the BCA reagent to form a stable blue-violet complex with strong absorption at 562 nm, and its absorbance value shows a good linear relationship with the protein concentration.
There are two modes of measurement available: standard concentration protein measurement (standard mode) (50 ~ 2000 μg/ml) and micro concentration protein measurement (micro mode) (<50 μg/ml), so please select the appropriate mode according to your experimental needs.
This product can be used in microplate and cuvette method for detection, microplate method is easy and fast to operate, only a small amount of protein samples; cuvette method requires a relatively large number of protein samples, cuvette method is longer than the optical diameter of the microplate, so the sensitivity will be better than the microplate method.
This product is highly tolerant to interfering substances such as surfactant SDS, Triton X-100, Tween-20, etc. in protein samples, but it is not suitable for quantification of protein samples containing reducing agents or chelating agents. The maximum tolerated concentrations of different interfering substances in protein samples are shown in Appendix 1.
1, the product has high sensitivity, wide linear range, absorbance value in the range of 5 ~ 2000 μg / ml protein concentration has a good linear relationship, but the lower limit of the concentration of different protein detection may be different.
2, the product is highly compatible, can be compatible with the determination of constant and micro protein samples at the same time.
3、This product has strong anti-interference performance and is resistant to many kinds of chemical substances.
| Component*1 | norm |
| BCA Reagent A | 100 ml |
| BCA Reagent B | 3 ml |
| BSA Standard Solution (2 mg/ml) | 1 ml X 3 pcs |
*1: BCA Reagent A and BCA Reagent B are packaged in Package 2-1 and stored at room temperature (15~30℃), and BSA Standard Solution (2 mg/ml) is packaged in Package 2-2 and stored at 4℃.
*2: Using the microplate method (200 μl reaction system), this product can perform 500 reactions.
Preservation Temperature:
Package 2-1: Store at room temperature (15 ~ 30°C);
Package 2-2: Store at 4°C;
Transportation temperature:
Package 2-1: Transportation at room temperature;
Package 2-2: Transportation at 4°C.
