Product Description
Pro Taq HS DNA Polymerase is an application of the LA PCR principle, which is performed on our superior Accurate Taq A high fidelity enzyme has been added to the enzyme to provide 3′→5′Exonuclease activity (Proof reading activity), which is ideal for amplification of DNA fragments over 10 kb and has good fidelity. A monoclonal antibody to Taq enzyme is also included in the system for Hot Start PCR, which binds to and inhibits Taq enzyme before the reaction starts, thus inhibiting non-specific amplification caused by primer annealing or primer dimerization at low temperatures. The antibody is inactivated during the initial DNA denaturation step when the PCR reaction is initiated, so it can be used under conventional PCR reaction conditions. Most PCR products amplified with this product have an A base at the 3’ end and can be cloned directly into T vectors.
Product Advantages
①High-fidelity enzyme is added to this product, relative to the Accurate Taq Has higher fidelity. High amplification efficiency and low mismatch rate under normal PCR amplification conditions.
② A monoclonal antibody that inhibits the activity of the Taq enzyme is added to this product, enabling a hot start reaction. The antibody binds to the enzyme and inhibits Taq enzyme activity prior to the PCR reaction, effectively inhibiting the formation of primer dimers and non-specific amplification.
③ This product can amplify ~40 kb of DNA fragments using λDNA as a template and ~20 kb of DNA fragments using Human gDNA as a template.
Product Composition
| individual parts making up a compound | norm |
| Pro Taq HS DNA Polymerase (5 U/μl) | 50 μl |
| 10X Pro Taq PCR Buffer Ver.2 (Mg)2+ plus) | 1 ml |
| dNTP Mix (10 mM each) | 200 μl |
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag
Transportation temperature: dry ice or -20℃ ice bag
