Accurate Super Taq HS DNA polymerase I

Product Code: CM0268

- +

¥1,500

Product Description

this product Accurate Super Taq HS DNA Polymerase I is genetically modified to provide high thermal stability, excellent amplification efficiency, amplification speed, amplification sensitivity and amplification specificity.
This product also contains an additive that can inhibit the production of chemical substances at room temperature. Accurate Super Taq The monoclonal antibody to DNA Polymerase I activity is optimized to maintain strict containment of the polymerase at 55°C, which can effectively inhibit non-specific amplification. When the reaction is kept at 95℃ for more than 30 sec, the monoclonal antibody is completely inactivated and the polymerase can be completely released, which makes the PCR system highly specific and sensitive, and improves the accuracy of the results.
this product Accurate Super Taq HS DNA Polymerase I has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity without 3’→5’ exonuclease activity and is suitable for a variety of Taq DNA Polymerase-based PCR and qPCR reactions. HS DNA Polymerase I has 5’→3' DNA polymerase activity and 5'→3' exonuclease activity without 3'→5' exonuclease activity, and is suitable for a wide range of Taq DNA Polymerase-based PCR and qPCR reactions; the PCR product obtained with this product has an A base at the 3' end, and can be cloned directly into T vector.

Product Advantages

1. High thermal stability: the half-life of heating at 95℃ is more than 1 hour.
2. High amplification efficiency: the extension time for fragments up to 1 kb is only 1 sec, and the extension time for fragments up to 2 kb can be shortened to 1 ~ 5 sec.

Product Composition
individual parts making up a compound norm
Accurate Super Taq HS DNA Polymerase I (30 U/μl) 50 μl
10X Super Taq HS PCR Buffer I (Mg2+ plus)* 1 ml x 3 pcs

*: 10X Super Taq HS PCR Buffer I (Mg2+ plus) composition: 100 mM Tris-HCl (pH 8.9 at 25°C), 500 mM KCl, 50 mM MgCl2

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature:Dry ice transportation or -20°C ice pack transportation.
Example 1

Accurate Super Taq HS DNA Polymerase I has a good amplification speed, using Human gDNA as a template, it can amplify DNA fragments of different lengths at a speed of up to 1 sec/kb.

The electrophoresis results are shown below:

Example 2

utilization Accurate Super Taq HS DNA Polymerase I amplifies DNA fragments of different lengths, ~3.4 kb using gDNA as template and ~10 kb using λDNA as template.

The electrophoresis results are shown below:

Example 3

Using λDNA as a template, different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg) were added using the Accurate Super Taq HS DNA Polymerase I amplified 2 kb of DNA fragments with a template amount as low as 10 fg and was still able to amplify the target band. The electrophoresis results are shown below:

Example 4

The African swine fever B646L standard [ GBW ( E ) 091034 ] was used as a template, and the B646L gene was detected by fluorescent probe qPCR method using this product, and the amount of template added to the 25 μl reaction system was 35 copies and 0 copies (negative control). The quantification instrument used was ABI QuantStudio.TM 5 Real-Time PCR Systems. the results of the experiments were as follows:

The results are shown above:
1, for low-copy virus templates, this product can be amplified well, the output rate is 100%.
2. Negative controls were not detected within 45 cycles.