<i>Accurate Super Taq</i> HS DNA聚合酶 II

Accurate Super Taq HS DNA Polymerase II

Product Code: CM0269

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¥2,250

Product Description

this product Accurate Super Taq HS DNA Polymerase II is genetically modified to provide high thermal stability, excellent amplification efficiency, anti-interference properties, amplification speed, amplification sensitivity and amplification specificity.
This product also contains an additive that can inhibit the production of chemical substances at room temperature. Accurate Super Taq The monoclonal antibody against DNA Polymerase II activity is optimized to maintain strict containment of the polymerase at 55℃, which can effectively inhibit non-specific amplification. When the reaction is kept at 95℃ for more than 30 sec, the monoclonal antibody is completely inactivated and the polymerase activity can be completely released, which makes the PCR system highly specific and sensitive, and improves the accuracy of the results.
this product Accurate Super Taq HS DNA Polymerase II has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity without 3’→5’ exonuclease activity and is suitable for a variety of Taq DNA Polymerase-based PCR and qPCR reactions. HS DNA Polymerase II has 5’→3' DNA polymerase activity and 5'→3' exonuclease activity without 3'→5' exonuclease activity, and is suitable for a wide range of Taq DNA Polymerase-based PCR and qPCR reactions; it produces a PCR product with an A base at the 3' end, which can be cloned directly into T vector.

Product Advantages

1. High thermal stability: the half-life of heating at 95℃ is more than 1 hour.
2. High amplification efficiency: the extension time for fragments up to 1 kb is only 1 sec, and the extension time for fragments up to 2 kb can be shortened to 1 ~ 5 sec.
3. Strong anti-interference performance: against different interfering substances (hemoglobin, EDTA, guanidinium isothiocyanate, ethanol, etc.), all have strong anti-inhibition ability.

Product Composition
individual parts making up a compound norm
Accurate Super Taq HS DNA Polymerase II (30 U/μl) 50 μl
10X Super Taq HS PCR Buffer II (Mg2+ plus)* 1 ml x 3 pcs

*: 10X Super Taq HS PCR Buffer II (Mg2+ plus) composition: 100 mM Tris-HCl (pH 8.9 at 25°C), 750 mM KCl, 30 mM MgCl2

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature:Dry ice transportation or -20°C ice pack transportation.
Example 1

Accurate Super Taq HS DNA Polymerase II has a good amplification speed, using Human gDNA as a template, it can amplify DNA fragments of different lengths at a speed of up to 1 sec/kb.

The electrophoresis results are shown below:

Example 2

utilization Accurate Super Taq HS DNA Polymerase II amplifies DNA fragments of different lengths, ~3.4 kb using gDNA as template and ~10 kb using λDNA as template.

The electrophoresis results are shown below:

Example 3

Using λDNA as a template, different template amounts (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg) were added using the Accurate Super Taq HS DNA Polymerase II amplified a 2 kb fragment of DNA with a template amount as low as 10 fg and was still able to amplify the target band. The electrophoresis results are shown below:

Example 4

Human cDNA was used as the template for RPP30 gene (GC content 62%, CY5 channel) quantitative weight detection, and different interfering substances were added, the final concentration and types of interfering substances in the system were: 40 μM Hemin, 4 mM EDTA, 6% Ethanol, 40 mM Guanidinium, Thiocyanate, 20 ng / μl Heparin Sodium, and the Ct difference between the amplification performance after adding interfering substances and the unadded group was less than 2, indicating that the product has a strong anti-interference ability. Thiocyanate, 20 ng / μl Heparin Sodium, the Ct difference between the amplification performance after the addition of interfering substances and that of the unadded group was less than 2, which indicated that the product had strong anti-interference ability, and the experimental results were as follows:

 

Example 5

The African swine fever B646L standard [ GBW ( E ) 091034 ] was used as a template, and the B646L gene was detected by fluorescent probe qPCR method using this product, and the amount of template added to the 25 μl reaction system was 35 copies and 0 copies (negative control). The quantification instrument used was ABI QuantStudio.TM 5 Real-Time PCR Systems. the results of the experiments were as follows:

The results are shown above:
1, for low-copy virus templates, this product can be amplified well, the output rate is 100%.
2. Negative controls were not detected within 45 cycles.

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