This product is designed to provide customers with a fast, simple, high-quality genomic DNA (gDNA) preparation method, based on high binding superparamagnetic magnetic beads and a unique buffer system, can be extracted from biological samples such as animal tissues and cultured cells to obtain a high yield of high-purity gDNA, the use of the product extracted from the gDNA can be used for PCR amplification, DNA sequence analysis, microarray detection, library construction and other experiments. The gDNA obtained by using this product can be directly used for PCR amplification, DNA sequence analysis, microarray detection and library construction. At the same time, this product can be used for single-tube manual extraction, and can also be used with automatic nucleic acid extractor to realize high-throughput extraction of nucleic acids.
| individual parts making up a compound | norm |
| RNase A Solution (10 mg / ml) | 1 ml |
| Proteinase K (20 mg / ml) | 1 ml |
| MagPure DNA Beads*2 | 1 ml |
| Mag Buffer LS*3 | 18 ml |
| Mag Buffer BS*3 | 18 ml |
| Mag Buffer DB*4 | 5 ml |
| Mag Buffer DWA*5 | 16 ml |
| RNase free water for 70% ethanol*6 | 21 ml |
| Mag Elution Buffe | 10 ml |
| RNase Free Water*7 | 10 ml |
*1: Components RNase A Solution (10 mg / ml) and Proteinase K (20 mg / ml) are packaged in Package 3-1 and stored at -20°C; Components MagPure The DNA Beads are packaged in Package 3-2 and stored at 4°C. The remaining components are packaged in Package 3-3 and stored at room temperature (15-30°C).
*2:MagPure DNA Beads should not be frozen or allowed to dry out, and should be vortexed and shaken before each use so that they are adequately suspended, as this may result in inconsistent amounts of beads being drawn up and thus affect the ability of the beads to bind DNA.
*3:Mag Buffer LS and Mag If precipitation occurs, heat Buffer BS at 50-60°C to dissolve and allow the solution to return to room temperature before use.
*4:Mag Before using Buffer DB for the first time, add 20 ml of isopropyl alcohol (Mag (Buffer DB to isopropanol volume ratio 1:4), mix well, mark the bottle and store at room temperature.
*5:Mag Before first use, add 24 ml of anhydrous ethanol to Buffer DWA (Mag Buffer DWA to anhydrous alcohol volume ratio of 2:3), mix well, mark the bottle and store at room temperature.
*6: RNase free water for 70% ethanol Before using for the first time, please add 49 ml of anhydrous ethanol (the volume ratio of RNase free water for 70% ethanol to anhydrous alcohol is 3:7) to formulate the ethanol for 70%, mix well and mark the bottle, store at room temperature. Mix well, mark the bottle and store it at room temperature.
*7: RNase Free Water is recommended to be stored at -20℃ after opening.
Preservation Temperature:
Package 3-1 -20°C Storage
Package 3-2 4°C storage
Package 3-3 Storage at room temperature (15-30°C)
Transportation temperature:
Package 3-1 Dry Ice or -20°C Ice Pack Transportation
Package 3-2 Room Temperature Transportation
Package 3-3 Room Temperature Transportation
