This product is derived from total RNA or poly(A).＋ A kit for the initial synthesis of 1st strand cDNA from RNA. Reverse transcriptase for 1st strand cDNA synthesis Evo Super M-MLV The plus, RNase Inhibitor, Oligo dTPrimer, dNTP Mixture and Reaction Buffer have been premixed into a Premix-type reagent, which is easy and fast to start the reaction by adding RNA template and water.
By adding auxiliary proteins and optimizing the Buffer composition, this product reduces the RNA denaturation operation before the reverse transcription reaction and makes the operation more convenient.
This product is characterized by high synthesis efficiency, fast reaction speed, high sensitivity, high thermal stability and strong resistance to inhibition, etc. It is able to synthesize 1st Strand cDNA more efficiently under the condition of 42℃~60℃; meanwhile, since the reverse transcription reaction can be carried out at high temperature, the synthesis of 1st Strand cDNA by using the Gene Specific Primer enhances the efficiency and specificity of reverse transcription. and specificity of 1st Strand cDNA synthesized with Gene Specific Primer.
The 1st strand cDNA synthesized in this product is suitable for 2nd strand cDNA synthesis, hybridization, PCR amplification, and preparation of full-length cDNA libraries. This product contains Random 6 mers, which can be used for the preparation of full-length cDNA libraries from poly(A)＋ It is also suitable for Real Time PCR for gene expression analysis.
1. Convenient operation: The pre-mixed solution has been prepared, only need to add the template RNA and water to the reaction, and the reagents will not freeze at -20 ℃, the reagents can be taken out of the refrigerator at -20 ℃ to carry out the reaction preparation.
2. Strong thermal stability: the reverse transcription reaction can be carried out under the condition of 42℃~60℃, and the high temperature is favorable for the synthesis of RNA template with complex secondary structure.
3. Impurity tolerance: good resistance to inhibitors remaining in the RNA (e.g., interfering substances such as guanidine isothiocyanate, ethanol, EDTA, sodium heparin and hemoglobin).
4. Wide range of use: the synthesized cDNA can be used for routine PCR and qPCR, and Random 6 mers are included in the product to synthesize cDNA from RNA that does not contain poly(A)+.
5. Highly transcriptional: can synthesize cDNA up to 12 kb in length.
6. Fast transcription: reverse transcription reaction can be completed within 5 min at the earliest.
| individual parts making up a compound | norm |
| 4X Evo Super M-MLV Plus cDNA Synthesis Mix*1 | 250 μl |
| Random 6 mers Primer (100 μM)*2 | 100 μl |
| RNase free water | 1 ml X 2 pcs |
*1: contains Evo Super M-MLV The reaction buffer was composed of RNase Inhibitor, Oligo dT(18T) Primer, dNTP Mixture, and reaction buffer.
*2: In the absence of poly(A)＋ This component should be added to the reaction solution for the synthesis of RNA start cDNA, the synthesis of cDNA for Real Time PCR, and the homogeneous synthesis of cDNA for the entire RNA region.
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
