<i>Evo M-MLV</i> 反转录试剂盒（用于 qPCR）

Evo M-MLV Reverse transcription kit (for qPCR)

Product Code: AG11707

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¥1,400

Product Description

This product is a reverse transcription reagent for Real Time RT-PCR. It utilizes a high elongation capacity Evo M-MLV Reverse transcriptase can synthesize cDNA in a short time, and the synthesized cDNA is compatible with chimeric and probe qPCR, which can be used for high-performance genetic analysis according to the purpose of the experiment with the appropriate reagents.

Product Advantages

The cDNA can be synthesized quickly and efficiently, and then analyzed by qPCR.
② The kit comes with two primers, Oligo dT (18T) Primer (50 μM) and Random 6 mers Primer (100 μM), which can be selected for reverse transcription according to the actual situation.
③ The cDNA synthesized by this kit is compatible with SYBR and probe qPCR, so you can select the appropriate reagents for use according to the purpose of the experiment and perform high-performance genetic analysis.

Product Composition
individual parts making up a compound norm
Evo M-MLV RTase Enzyme Mix *1 100 μl
5X RTase Reaction Buffer Mix II*2 400 μl
Oligo dT (18T) Primer (50 μM) 100 μl
Random 6 mers Primer (100 μM) 400 μl
RNase free water 1 ml X 2 pcs

*1: contains Evo M-MLV RTase, RNase Inhibitor.
*2: Contains dNTP.

Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

Mouse Total RNA of different gradients (2 μg~2 pg) was used as template for reverse transcription with this kit, and then 2 μl of this cDNA was used as template for the cDNA stock solution, and the cDNA stock solution was extracted with SYBR Green. Pro Taq HS Premixed qPCR Kit (Code. AG11701) for qPCR detection of mouse GAPDH Genes.

The results are shown above:
①Standard curve R2= 0.997, amplification efficiency 106.01 TP3T.
②This product has a high reversal efficiency and can be accurately quantified over a wide template range. The cDNA amplification curves obtained by reverse transcription of 2 μg ~ 2 pg Total RNA show good linearity.
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

Example 2

Total RNA of 293T cells with different gradients was used as template (2 μg~20 pg), reverse transcription was performed with this kit, and 2 μl of the cDNA stock solution was taken, and the cDNA was analyzed by SYBR Green. Pro Taq HS Premix qPCR kit (Code. AG11701) for qPCR detection Human H32F Genes.

The results are shown above:
①Standard curve R2= 0.999, amplification efficiency 103.11 TP3T.
②This product has a high reversal efficiency and can be accurately quantified over a wide template range. The cDNA amplification curves obtained by reverse transcription of 2 μg ~ 20 pg Total RNA show good linearity.
③ The melting curve has a single peak type, no spurious peaks, and strong amplification specificity.

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