This is a kit for miRNA first-strand cDNA synthesis using the A-tail method. The first-strand cDNA of miRNA is synthesized by adding A-tail at the 3’ end of miRNA using Poly ( A ) Polymerase, and then reverse transcription using universal reverse transcription primers (Oligo (dT) primers with specific tags). miRNA RT Enzyme Mix contains A-tail and reverse transcriptase, and 2X miRNA RT Reaction Solution contains universal reverse transcription primers, which are optimized to synthesize miRNA first-strand cDNA from the same kit. In this product, miRNA RT Enzyme Mix contains A-tailed enzyme and reverse transcriptase, and 2X miRNA RT Reaction Solution contains universal reverse transcription primers, which are optimized to complete the A-tailed and reverse transcription reactions at the same time in the same reaction system, which simplifies the process of experimental operation and reduces the loss of reagents and experimental errors. This product is suitable for reverse transcription of miRNA samples containing Total RNA or small RNA.
This product contains the primer miRNA qPCR 3’primer for subsequent qPCR assays.
① This kit combines the miRNA A tailing reaction and reverse transcription reaction into one, which is easy to use, reduces the experimental error and improves the detection rate of low abundance miRNAs.
② This kit can reverse transcribe samples containing miRNA such as Total RNA or small RNA.
| individual parts making up a compound | AG11716 | AG11717 |
| miRNA RT Enzyme Mix*1 | 12.5 μl | 62.5 μl |
| 2X miRNA RT Reaction Solution*2 | 50 μl | 250 μl |
| miRNA qPCR 3’primer (10 μM)*3 | 250 μl | 625 μl x 2 pcs |
| RNase free water | 1 ml | 1 ml |
*1: Contains Poly(A) Polymerase,M-MLV RTase, RNase Inhibitor;
*2: Contains universal reverse transcription primers;
*3: For qPCR reaction, Tm value is 59°C.
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
