2X Accurate Taq Premix (with dye, for PAGE)

Product Code: AG11023

- +

¥140

Product Description
this product is Accurate Taq DNA polymerase ready-to-use 2-fold PCR reaction premixes containing Accurate Taq DNA Polymerase, dNTPs and optimized Buffer system, PCR reaction, just add the template, primers and water to the premixed solution can be amplified. At the same time, the product is also added with the color reagent required for electrophoresis testing, the solution of the product is purple-red in color, and can be used for polyacrylamide gel electrophoresis or agarose gel electrophoresis directly after the PCR reaction is completed. The premixed solution is easy to use, minimizing human error and contamination from multiple steps, and results can be obtained in a short period of time. The PCR product has an A base at the 3’ end, and can be purified and cloned directly into T vectors.
Product Advantages
The product is optimized for polyacrylamide gel electrophoresis, so that PCR products can be directly applied to polyacrylamide gel electrophoresis or agarose gel electrophoresis. The product contains violet dye, which eliminates the need for electrophoresis buffer, and results in a light violet-red color band during electrophoresis.
② The product is a 2X premix, only need to add template, primers and water to the premix for amplification, easy to operate, can minimize human error, reduce the possible contamination of multi-step operation, and can obtain the test results in a relatively short period of time.
③ 2X Accurate Taq Master Mix (dye plus, for PAGE) has good amplification performance, amplifying ~12 kb of DNA fragments using λDNA as a template and ~4 kb of DNA fragments using Human genomic DNA as a template.
Product Composition
individual parts making up a compound norm
2X Accurate Taq Master Mix (dye plus, for PAGE) 500 μl x 6 pcs
RNase free water 1 ml x 3 pcs
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag
Example 1

Chicken gDNA, pig gDNA and human gDNA were used as templates to amplify different lengths of DNA fragments, and good amplification results were obtained.

The results of polyacrylamide gel electrophoresis are shown below:

Example 2

Human gDNA was used as a template for the multiplex PCR reaction using this kit, and different lengths of DNA fragments (0.3 kb / 0.5 kb / 1 kb / 2 kb) were amplified with good results.

The results of agarose gel electrophoresis are shown below: