2X L-Exp Taq HS PCR Premix

Product Code: AG11514

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¥550

Product Description

This product is ready to use L-Exp Taq Enzyme PCR reaction 2-fold pre-mix containing L-Exp Taq HS DNA Polymerase, dNTPs, and optimized Buffer system, for PCR reaction, just add template, primers and water to the premix for amplification. This premix solution is easy to use, minimizes human error, reduces contamination caused by multiple steps, and results can be obtained in a short period of time.
included L-Exp Taq The enzyme is a carefully optimized DNA PCR reaction system that utilizes the principles of LA PCR and is ideal for PCR amplification of long fragments and complex DNA sequences. The enzyme system also includes a monoclonal antibody to Taq for Hot Start PCR, which binds to and inhibits the Taq enzyme prior to the start of the reaction, thus inhibiting non-specific amplification caused by primer annealing or primer dimerization at low temperatures. The antibody is inactivated during the initial DNA denaturation step when the PCR reaction is initiated, so it can be used under routine PCR reaction conditions. Most PCR products obtained with this product have an A base at the 3’ end and can be cloned directly into T vectors.

Product Advantages
① The product is a 2X premix, only need to add template, primer and water to the premix for amplification, easy to operate, can minimize human error, reduce the possible contamination caused by multi-step operation, and the results can be obtained in a relatively short period of time.
② This product has very strong long fragment amplification performance, and can amplify ~24 kb of DNA fragments using Human genomic DNA as template.
③ This product has the ability to amplify complex templates and has a high amplification success rate for higher GC content templates (~75%) that are difficult to amplify.
④ A monoclonal antibody that can inhibit the activity of DNA polymerase has been added to this product, which can be used for hot start reaction. Before PCR reaction, the antibody binds to the enzyme and inhibits the activity of DNA polymerase, effectively inhibiting the formation of primer dimer and non-specific amplification.
Product Composition
individual parts making up a compound norm
2X L-Exp Taq HS PCR Master Mix 500 μl / 3 pc
RNase free water 1 ml x 2 pc
Preservation and transportation
Storage temperature: -20℃
Transportation temperature: dry ice or -20℃ ice bag transportation
Example 1

Using λDNA as a template, the kit amplifies DNA fragments of different lengths, and is able to amplify 40 kb of DNA fragments.

The electrophoresis results are as follows:

Example 2

Using human gDNA as a template, this kit amplifies DNA fragments of different lengths, and can well amplify 24 kb DNA fragments.

The electrophoresis results are as follows: