RACE (Rapid Amplification of cDNA Ends) is a technique for rapid amplification of cDNA ends of unknown genes, starting from known sequences in some regions. This product was developed using Total RNA or Poly A+ A kit for rapid amplification of cDNA 5’ end and 3’ end sequences by reverse transcription using RNA as a template. The kits contain the components required for 5’ RACE and 3’ RACE amplification and can be selected according to needs.
For 5’ RACE, the template-switching activity of reverse transcriptase is utilized to add a splice at the 5’ end of the RNA, and RACE PCR amplification can be carried out directly without the need for Adaptor connection and other steps, making the entire process faster and simpler; PCR The PCR amplification system utilizes our high performance L-Exp Taq HS DNA Polymerase, with the ability to amplify complex fragments such as long fragments or high GC content, and carefully optimized Buffer system, this product has a high degree of specificity, which can reduce the dispersion phenomenon in RACE experiments.
This product contains all the reagents for the carefully optimized 5’ RACE and 3’ RACE reactions, please use the reagents included in this kit for reverse transcription and PCR amplification experiments, and if you need to make substitutions, please verify them first.
| Component (Package 2-1, stored at -80°C) | norm |
| Template Switching Oligo (TSO) (24 µM) | 10 μl |
| Control Mouse Liver Total RNA (1 µg /μl) | 10 μl |
| Components (Package 2-2, stored at -20°C) | norm |
| 3’RACE RT Primer (12 µM) | 10 μl |
| 5’ RACE RT Primer (12 µM) | 10 μl |
| Random Primer (20 µM) | 10 μl |
| RNase Inhibitor (40 U/μl) | 10 μl |
| 5X RACE RT Buffer | 40 μl |
| Evo M-MLV RTase for RACE (100U /μl) | 20 μl |
| dNTP Mix (10 mM each) | 120 μl |
| 10X Universal Primer Mix | 400 μl |
| Tricine EDTA Buffer | 1 ml X 2 pcs |
| Universal Short Primer (10 µM) | 50 μl |
| Control 5′-Gene-Specific Primer (10 µM)* | 25 μl |
| Control 3′-Gene-Specific Primer (10 µM)* | 25 μl |
| L-Exp Taq HS DNA Polymerase (5U /μl) | 25 μl |
| 5X L-Exp Taq PCR Buffer (Mg2+ plus) | 500 μl |
| Nuclease free water | 1 ml |
*: DNA fragments amplified with Control 5′-Gene-Specific Primer were 1.6 kb in length; DNA fragments amplified with Control 3′-Gene-Specific Primer were 0.6 kb in length.
2,Evo M-MLV RTase for RACE has strong reverse transcription ability, and can reverse long fragments and complex structural templates well;
Storage temperature: Package 2-1 -80℃ Storage Package 2-2 -20℃ Storage
Transportation Temperature: Package 2-1 Dry Ice Transportation (Avoid repeated freezing and thawing) Package 2-2 -20°C Ice Pack Transportation or Dry Ice Transportation
