AccuFect Cell transfection kit (nanopolymer method)

Product Code: AG51003

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¥980

Product Description

This product is a transfection reagent composed of nanopolymers for transfection of plasmid DNA into eukaryotic cells. The principle is that plasmid DNA and nanopolymer form a multimeric complex, attach to the surface of the cell membrane, and enter into the cell by endocytosis, transcription and expression in the cell. This method is characterized by high transfection efficiency, good reproducibility, simple operation and no obvious cytotoxicity for common mammalian cells. The product is basically unaffected by the presence of serum and antibiotics in the cell culture medium, and can be used to transfect cells in the presence of serum and antibiotics.

Product Advantages

①This product has high transfection efficiency, good reproducibility and no obvious cytotoxicity;
②This product has wide applicability and is suitable for most of the adherent cells;
③This product is not affected by serum and antibiotics, so there is no need to change to serum-free medium during transfection;
④This product is not only suitable for transient transfection of cells, but also for stable transfection of cells.

Product Composition
individual parts making up a compound norm
NanoFect Reagent 150 μl X 2 pcs
NanoFect Buffer 5 ml X 2 pcs
Preservation and transportation
Storage temperature: -20℃ storage (NanoFect Reagent After each use, tighten the cap of the tube and return it to the bag containing the desiccant.)
Transportation temperature: dry ice transportation or -20℃ ice bag transportation
Example 1

In a 12-well plate, 2 μg of EGFP plasmid was transfected into 293T cells and 293 cells using this product, and after 48 h, strong green fluorescence could be observed under the microscope, and high transfection efficiency was obtained, as shown in the figure below: