Product Description
It is a transposase library kit developed for illumina's high-throughput sequencing platform, which can prepare sequencing libraries from purified 1 ng ~ 100 ng DNA samples. Using a novel transposase enzyme and optimized Buffer system, DNA fragmentation and junction ligation can be completed in just 10 min, which is much simpler and more convenient than traditional library preparation methods, and eliminates the need for tedious DNA fragmentation, end repair and junction ligation reactions, thus significantly shortening the time for library construction. Since Transposome Mix is very sensitive to the amount of DNA added, the exact amount of DNA added is crucial to the success of the experiment. Therefore, we have optimized the relationship between the amount of Transposome Mix and the amount of DNA, and developed four kits with different template starting amounts for different DNA template additions, namely 100 ng (Code No. AG12518 / AG12519), 50 ng (Code No. AG12520 / AG12521), 5 ng (Code No. AG12520 / AG12521), 5 ng (Code No. AG12520 / AG12521), and 5 ng (Code No. AG12520 / AG12521). ng (Code No. AG12522 / AG12523), 1 ng (Code No. AG12524 / AG12525), which can be selected according to needs.
The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change the dosage and concentration of any reaction components or to replace the components in this product with other equivalent products in order to avoid obtaining bad results. If replacement is needed, please verify it first.
The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change the dosage and concentration of any reaction components or to replace the components in this product with other equivalent products in order to avoid obtaining bad results. If replacement is needed, please verify it first.
Product Advantages
1. Using a new transposase and optimized reaction system, DNA fragmentation and ligation steps can be completed in only 10 min, which significantly shortens the library construction time, and library construction can be completed in less than 1.5 hours, and the library quality is excellent.
2. Simple operation, single-tube enzymatic reaction for fragmentation and splicing without mechanical interruption.
3. Low DNA template incorporation, down to 1 ng of DNA template.
4. Wide applicability of templates, 4 different template quantities (100 ng / 50 ng / 5 ng / 1 ng) have been designed for selection according to needs.
2. Simple operation, single-tube enzymatic reaction for fragmentation and splicing without mechanical interruption.
3. Low DNA template incorporation, down to 1 ng of DNA template.
4. Wide applicability of templates, 4 different template quantities (100 ng / 50 ng / 5 ng / 1 ng) have been designed for selection according to needs.
Product Composition
| individual parts making up a compound | AG12520 | AG12521 |
| AccuNext Transposome Mix V50*a | 12 μl | 48 μl |
| 5X Tn5 Reaction Buffer*b | 48 μl | 192 μl |
| 5X Stop Buffer*b | 60 μl | 240 μl |
| AccuNext PCR Mix*b | 324 μl | 1.3 ml |
*a:AccuNext Transposome Mix V50 should be stored at -80°C; for 50 ng of DNA template, do not adjust the amount of template added.
*b: 5X Tn5 Reaction Buffer, 5X Stop Buffer and AccuNext PCR Mix can be stored at -80°C or -20°C.
*c: illumina sequencing junction primers for PCR amplification are required for the experiment but are not configured in this product and need to be purchased separately. For example, AccuNext Transposon Building Block Adaptor Primers (Illumina) (Code No. AG12532, AG12533, AG12534) can be used].
Preservation and transportation
Storage temperature: -80℃ storage
Transportation temperature: dry ice transportation
Transportation temperature: dry ice transportation
