Product Description
This product is a new generation of high-efficiency DNA cyclization kit tailor-made for the UW-MGI high-throughput sequencing platform, and is suitable for all amplification products of libraries with MGI standard double-label PCR junctions attached. The optimized reaction system and high-quality enzyme components significantly improve the cyclization efficiency and success rate of DNA templates.
All reagents provided in this product have been subjected to strict quality control and functional validation to maximize the stability and reproducibility of the library construction, therefore, it is not recommended to change the dosage and concentration of any reaction components or replace the components in this product with other equivalent products to avoid obtaining undesirable experimental results. If replacement is needed, please verify it first.
All reagents provided in this product have been subjected to strict quality control and functional validation to maximize the stability and reproducibility of the library construction, therefore, it is not recommended to change the dosage and concentration of any reaction components or replace the components in this product with other equivalent products to avoid obtaining undesirable experimental results. If replacement is needed, please verify it first.
Product Advantages
1. Low starting volume requirement: support 0.2 pmol input volume, reduce sample consumption cost, broaden the precious sample application scene.
2. Minimal and fast operation: individual steps take ≤ 10 minutes.
2. Minimal and fast operation: individual steps take ≤ 10 minutes.
Product Composition
| individual parts making up a compound | norm |
| DB Splint Oligo | 80 μl |
| Splint Buffer | 80 μl |
| Ligase | 80 μl |
| Digestion Buffer | 96 μl |
| Digestion Enzyme | 64 μl |
Preservation and transportation
Storage temperature: -20℃ storage
Transportation temperature: -20℃ ice bag transportation
Transportation temperature: -20℃ ice bag transportation
