AccuNext Rapid DNA Library Preparation Kit Ver.2 (Illumina, 50~100 ng template volume)

It is a transposase DNA library kit developed for illumina's high-throughput sequencing platform, which can prepare sequencing libraries from purified 1 ng ~ 100 ng DNA samples. Using a new transposase enzyme and optimized buffer system, DNA fragmentation and junction ligation can be completed in only 10 minutes, which is much simpler and more convenient than traditional library preparation methods, and eliminates the need for tedious DNA fragmentation, end repair and junction ligation reactions, thus significantly shortening the time for library construction. Since Transposome Mix is very sensitive to the amount of DNA added, the exact amount of DNA added is crucial to the success of the experiment. Therefore, this product has optimized the relationship between the amount of Transposome Mix and DNA, and developed three kits with different template starting amounts for different DNA template incorporation amounts, namely 50~100 ng (Code No. AG12518 / AG12519), 5 ng (Code No. AG12522 / AG12523), 1 ng (Code No. AG12522 / AG12523), 1 ng (Code No. AG12523), 1 ng (Code No. AG12524 / AG1252519), and 1 ng (Code No. AG1252519). (Code No. AG12518 / AG12519), 5 ng (Code No. AG12522 / AG12523), and 1 ng (Code No. AG12524 / AG12525), which can be selected according to needs.
The reaction system of this product has been carefully optimized, all reagents in the experiment should be used as provided in this product, it is not recommended to change the dosage and concentration of any reaction components or to replace the components in this product with other equivalent products in order to avoid obtaining bad results. If replacement is needed, please verify it first.

1. Using a new transposase and optimized reaction system, DNA fragmentation and ligation steps can be completed in only 10 min, which significantly shortens the library construction time, and library construction can be completed in less than 1.5 hours, and the library quality is excellent.
2. Simple operation, single-tube enzymatic reaction for fragmentation and splicing without mechanical interruption.
3. Low DNA template incorporation, down to 1 ng of DNA template.
4. Wide applicability of templates, 4 different template quantities (50~100 ng / 5 ng / 1 ng) have been designed and can be selected according to the needs.

individual parts making up a compound	AG12518	AG12519
AccuNext Transposome Mix V100*a	24 μl	96 μl
5X Tn5 Reaction Buffer*b	48 μl	192 μl
5X Stop Buffer*b	60 μl	240 μl
AccuNext PCR Mix II*b	240 μl	960 μl
Nuclease free water	1 ml	1 ml X 2 pcs

*a:AccuNext Transposome Mix V100 needs to be stored at -80°C;AccuNext Transposome Mix V100 is suitable for DNA template amounts of 50 to 100 ng, and the amount of DNA template can be adjusted within this range.
*b: 5X Tn5 Reaction Buffer, 5X Stop Buffer and AccuNext PCR Mix II can be stored at -80°C or -20°C.
*c: illumina sequencing junction primers for PCR amplification are required for the experiment but are not configured in this product and need to be purchased separately. [For example, the AccuNext Transposome building library adaptor primers (Illumina) (Code No. AG12532 / AG12533 / AG12534)].

Storage temperature: -80℃ storage
Transportation temperature: dry ice transportation